---
title: "Airway microbiome-metabolome signature linked to pediatric severe asthma"
id: "pubmed-42758777"
canonical_url: "https://medichelpline.com/clinical-feed/pubmed-42758777"
content_type: "clinical_feed_article"
specialty: "Critical Care"
source_name: "PubMed / NCBI"
source_url: "https://pubmed.ncbi.nlm.nih.gov/42758777/"
doi: "10.1371/journal.pone.0358560"
published_at: "2026-09-18T00:00:00.000Z"
evidence_level: "Journal Article"
license: "CC-BY-NC-4.0 / Informational Use"
---
# Airway microbiome-metabolome signature linked to pediatric severe asthma
## Provenance & Clinical Metadata
- **Canonical URL:** https://medichelpline.com/clinical-feed/pubmed-42758777
- **Specialty:** [Critical Care](https://medichelpline.com/clinical-feed/critical-care.md)
- **Primary Source:** PubMed / NCBI
- **Source URL:** [Original Journal Publication](https://pubmed.ncbi.nlm.nih.gov/42758777/)
- **DOI:** [10.1371/journal.pone.0358560](https://doi.org/10.1371%2Fjournal.pone.0358560)
- **Published At:** 2026-09-18T00:00:00.000Z
- **Evidence Rating:** Journal Article
## Executive GIST (TL;DR)
- The study used paired **microbiome** (16S rRNA gene amplicon sequencing) and **metabolome** (LC-HRMS) analyses of bronchoalveolar lavage (BAL) fluid to identify local signatures of pediatric severe asthma. - Samples comprised 20 children with severe asthma and 10 non-asthmatic disease-control children; analyses were performed separately and with integrative multi-omics methods. - BALs from children with severe asthma showed increased **alpha-diversity** and higher relative abundances of Actinobacteriota, **Streptococcus**, Moraxella, Corynebacterium, Tropheryma, and Treponema compared with controls. - Metabolomics revealed alterations in the **polyamine pathway**, specifically reduced arginine levels and elevated **spermine** and **spermidine** in severe asthma BALs. - Integrated analyses found significant associations between **Streptococcus** abundance and both spermine and spermidine levels, suggesting microbial–metabolic interactions in the airways. - Each dataset (microbiome alone, metabolome alone) could discriminate severe asthma phenotypes, notably exacerbation frequency and presence of co-occurring atopic dermatitis. - Unsupervised clustering of microbiome profiles identified four distinct clusters that the authors suggest may correspond to different severe asthma endotypes. - The findings support the potential of multi-omics BAL profiling for refining diagnosis, endotyping, and follow-up in pediatric severe asthma; detailed methodological or outcome limitations beyond the abstract were not reported in the source.
## Clinical Analysis & Structured Key Points
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A microbiome-metabolome signature associated with pediatric severe asthma [Mélanie Briard](https://pubmed.ncbi.nlm.nih.gov/?term=Briard+M&cauthor_id=42758777)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#full-view-affiliation-1 "Université Paris-Saclay, CEA, INRAE, UMR Département Médicaments et Technologies pour la Santé \(DMTS\)/SPI/Laboratoire d'Immuno-Allergie Alimentaire, Gif-sur-Yvette, France.")[ 2 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#full-view-affiliation-2 "Université Paris-Saclay, INRAE, AgroParisTech, UMR1319 Micalis Institute, Jouy-en-Josas, France."), [Blanche Guillon](https://pubmed.ncbi.nlm.nih.gov/?term=Guillon+B&cauthor_id=42758777)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#full-view-affiliation-1 "Université Paris-Saclay, CEA, INRAE, UMR Département Médicaments et Technologies pour la Santé \(DMTS\)/SPI/Laboratoire d'Immuno-Allergie Alimentaire, Gif-sur-Yvette, France."), [Eric Venot](https://pubmed.ncbi.nlm.nih.gov/?term=Venot+E&cauthor_id=42758777)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#full-view-affiliation-1 "Université Paris-Saclay, CEA, INRAE, UMR Département Médicaments et Technologies pour la Santé \(DMTS\)/SPI/Laboratoire d'Immuno-Allergie Alimentaire, Gif-sur-Yvette, France."), [Marta Grauso](https://pubmed.ncbi.nlm.nih.gov/?term=Grauso+M&cauthor_id=42758777)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#full-view-affiliation-1 "Université Paris-Saclay, CEA, INRAE, UMR Département Médicaments et Technologies pour la Santé \(DMTS\)/SPI/Laboratoire d'Immuno-Allergie Alimentaire, Gif-sur-Yvette, France."), [Christelle Hennequet-Antier](https://pubmed.ncbi.nlm.nih.gov/?term=Hennequet-Antier+C&cauthor_id=42758777)[ 3 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#full-view-affiliation-3 "INRAE, Université Paris-Saclay, BioinfOmics, MIGALE Bioinformatics Facility, Jouy-en-Josas, France.")[ 4 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#full-view-affiliation-4 "Université Paris-Saclay, INRAE, MaIAGE, 78350Jouy-en-Josas, France."), [Aurélia Bruneau](https://pubmed.ncbi.nlm.nih.gov/?term=Bruneau+A&cauthor_id=42758777)[ 2 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#full-view-affiliation-2 "Université Paris-Saclay, INRAE, AgroParisTech, UMR1319 Micalis Institute, Jouy-en-Josas, France."), [François Fenaille](https://pubmed.ncbi.nlm.nih.gov/?term=Fenaille+F&cauthor_id=42758777)[ 5 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#full-view-affiliation-5 "Université Paris-Saclay, CEA, INRAE, UMR Département Médicaments et Technologies pour la Santé \(DMTS\)/SPI/Laboratoire innovations en spectrométrie de masse pour la santé, MetaboHUB, Gif-sur-Yvette, France."), [Florence Castelli](https://pubmed.ncbi.nlm.nih.gov/?term=Castelli+F&cauthor_id=42758777)[ 5 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#full-view-affiliation-5 "Université Paris-Saclay, CEA, INRAE, UMR Département Médicaments et Technologies pour la Santé \(DMTS\)/SPI/Laboratoire innovations en spectrométrie de masse pour la santé, MetaboHUB, Gif-sur-Yvette, France."), [Muriel Thomas](https://pubmed.ncbi.nlm.nih.gov/?term=Thomas+M&cauthor_id=42758777)[ 2 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#full-view-affiliation-2 "Université Paris-Saclay, INRAE, AgroParisTech, UMR1319 Micalis Institute, Jouy-en-Josas, France."), [Guillaume Lezmi](https://pubmed.ncbi.nlm.nih.gov/?term=Lezmi+G&cauthor_id=42758777)[ 6 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#full-view-affiliation-6 "AP-HP, Hôpital Necker-Enfants Malades, Service de Pneumologie et Allergologie Pédiatriques, Paris, France."), [Maria Leite-de-Moraes](https://pubmed.ncbi.nlm.nih.gov/?term=Leite-de-Moraes+M&cauthor_id=42758777)[ 7 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#full-view-affiliation-7 "Université Paris Cité, Institut Necker Enfants Malades, Equipe Immunorégulation et Immunopathologie, Inserm UMR1151, CNRS, Paris, France."), [Karine Adel-Patient](https://pubmed.ncbi.nlm.nih.gov/?term=Adel-Patient+K&cauthor_id=42758777)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#full-view-affiliation-1 "Université Paris-Saclay, CEA, INRAE, UMR Département Médicaments et Technologies pour la Santé \(DMTS\)/SPI/Laboratoire d'Immuno-Allergie Alimentaire, Gif-sur-Yvette, France."), [Vinciane Saint-Criq](https://pubmed.ncbi.nlm.nih.gov/?term=Saint-Criq+V&cauthor_id=42758777)[ 2 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#full-view-affiliation-2 "Université Paris-Saclay, INRAE, AgroParisTech, UMR1319 Micalis Institute, Jouy-en-Josas, France.") Affiliations Expand ### Affiliations * 1 Université Paris-Saclay, CEA, INRAE, UMR Département Médicaments et Technologies pour la Santé (DMTS)/SPI/Laboratoire d'Immuno-Allergie Alimentaire, Gif-sur-Yvette, France. * 2 Université Paris-Saclay, INRAE, AgroParisTech, UMR1319 Micalis Institute, Jouy-en-Josas, France. * 3 INRAE, Université Paris-Saclay, BioinfOmics, MIGALE Bioinformatics Facility, Jouy-en-Josas, France. * 4 Université Paris-Saclay, INRAE, MaIAGE, 78350Jouy-en-Josas, France. * 5 Université Paris-Saclay, CEA, INRAE, UMR Département Médicaments et Technologies pour la Santé (DMTS)/SPI/Laboratoire innovations en spectrométrie de masse pour la santé, MetaboHUB, Gif-sur-Yvette, France. * 6 AP-HP, Hôpital Necker-Enfants Malades, Service de Pneumologie et Allergologie Pédiatriques, Paris, France. * 7 Université Paris Cité, Institut Necker Enfants Malades, Equipe Immunorégulation et Immunopathologie, Inserm UMR1151, CNRS, Paris, France. * PMID: **42758777** * DOI: [ 10.1371/journal.pone.0358560 ](https://doi.org/10.1371/journal.pone.0358560) Item in Clipboard # A microbiome-metabolome signature associated with pediatric severe asthma Mélanie Briard et al. PLoS One. 2026. Show details Display options Display options Format Abstract PubMed PMID PLoS One Actions * [ Search in PubMed ](https://pubmed.ncbi.nlm.nih.gov/?term=%22PLoS+One%22%5Bjour%5D&sort=date&sort_order=desc) * [ Search in NLM Catalog ](https://www.ncbi.nlm.nih.gov/nlmcatalog?term=%22PLoS+One%22%5BTitle+Abbreviation%5D) * [ Add to Search ](https://pubmed.ncbi.nlm.nih.gov/42758777/) . 2026 Sep 18;21(9):e0358560. doi: 10.1371/journal.pone.0358560. eCollection 2026. ### Authors [Mélanie Briard](https://pubmed.ncbi.nlm.nih.gov/?term=Briard+M&cauthor_id=42758777)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#short-view-affiliation-1 "Université Paris-Saclay, CEA, INRAE, UMR Département Médicaments et Technologies pour la Santé \(DMTS\)/SPI/Laboratoire d'Immuno-Allergie Alimentaire, Gif-sur-Yvette, France.")[ 2 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#short-view-affiliation-2 "Université Paris-Saclay, INRAE, AgroParisTech, UMR1319 Micalis Institute, Jouy-en-Josas, France."), [Blanche Guillon](https://pubmed.ncbi.nlm.nih.gov/?term=Guillon+B&cauthor_id=42758777)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#short-view-affiliation-1 "Université Paris-Saclay, CEA, INRAE, UMR Département Médicaments et Technologies pour la Santé \(DMTS\)/SPI/Laboratoire d'Immuno-Allergie Alimentaire, Gif-sur-Yvette, France."), [Eric Venot](https://pubmed.ncbi.nlm.nih.gov/?term=Venot+E&cauthor_id=42758777)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#short-view-affiliation-1 "Université Paris-Saclay, CEA, INRAE, UMR Département Médicaments et Technologies pour la Santé \(DMTS\)/SPI/Laboratoire d'Immuno-Allergie Alimentaire, Gif-sur-Yvette, France."), [Marta Grauso](https://pubmed.ncbi.nlm.nih.gov/?term=Grauso+M&cauthor_id=42758777)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#short-view-affiliation-1 "Université Paris-Saclay, CEA, INRAE, UMR Département Médicaments et Technologies pour la Santé \(DMTS\)/SPI/Laboratoire d'Immuno-Allergie Alimentaire, Gif-sur-Yvette, France."), [Christelle Hennequet-Antier](https://pubmed.ncbi.nlm.nih.gov/?term=Hennequet-Antier+C&cauthor_id=42758777)[ 3 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#short-view-affiliation-3 "INRAE, Université Paris-Saclay, BioinfOmics, MIGALE Bioinformatics Facility, Jouy-en-Josas, France.")[ 4 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#short-view-affiliation-4 "Université Paris-Saclay, INRAE, MaIAGE, 78350Jouy-en-Josas, France."), [Aurélia Bruneau](https://pubmed.ncbi.nlm.nih.gov/?term=Bruneau+A&cauthor_id=42758777)[ 2 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#short-view-affiliation-2 "Université Paris-Saclay, INRAE, AgroParisTech, UMR1319 Micalis Institute, Jouy-en-Josas, France."), [François Fenaille](https://pubmed.ncbi.nlm.nih.gov/?term=Fenaille+F&cauthor_id=42758777)[ 5 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#short-view-affiliation-5 "Université Paris-Saclay, CEA, INRAE, UMR Département Médicaments et Technologies pour la Santé \(DMTS\)/SPI/Laboratoire innovations en spectrométrie de masse pour la santé, MetaboHUB, Gif-sur-Yvette, France."), [Florence Castelli](https://pubmed.ncbi.nlm.nih.gov/?term=Castelli+F&cauthor_id=42758777)[ 5 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#short-view-affiliation-5 "Université Paris-Saclay, CEA, INRAE, UMR Département Médicaments et Technologies pour la Santé \(DMTS\)/SPI/Laboratoire innovations en spectrométrie de masse pour la santé, MetaboHUB, Gif-sur-Yvette, France."), [Muriel Thomas](https://pubmed.ncbi.nlm.nih.gov/?term=Thomas+M&cauthor_id=42758777)[ 2 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#short-view-affiliation-2 "Université Paris-Saclay, INRAE, AgroParisTech, UMR1319 Micalis Institute, Jouy-en-Josas, France."), [Guillaume Lezmi](https://pubmed.ncbi.nlm.nih.gov/?term=Lezmi+G&cauthor_id=42758777)[ 6 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#short-view-affiliation-6 "AP-HP, Hôpital Necker-Enfants Malades, Service de Pneumologie et Allergologie Pédiatriques, Paris, France."), [Maria Leite-de-Moraes](https://pubmed.ncbi.nlm.nih.gov/?term=Leite-de-Moraes+M&cauthor_id=42758777)[ 7 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#short-view-affiliation-7 "Université Paris Cité, Institut Necker Enfants Malades, Equipe Immunorégulation et Immunopathologie, Inserm UMR1151, CNRS, Paris, France."), [Karine Adel-Patient](https://pubmed.ncbi.nlm.nih.gov/?term=Adel-Patient+K&cauthor_id=42758777)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#short-view-affiliation-1 "Université Paris-Saclay, CEA, INRAE, UMR Département Médicaments et Technologies pour la Santé \(DMTS\)/SPI/Laboratoire d'Immuno-Allergie Alimentaire, Gif-sur-Yvette, France."), [Vinciane Saint-Criq](https://pubmed.ncbi.nlm.nih.gov/?term=Saint-Criq+V&cauthor_id=42758777)[ 2 ](https://pubmed.ncbi.nlm.nih.gov/42758777/#short-view-affiliation-2 "Université Paris-Saclay, INRAE, AgroParisTech, UMR1319 Micalis Institute, Jouy-en-Josas, France.") ### Affiliations * 1 Université Paris-Saclay, CEA, INRAE, UMR Département Médicaments et Technologies pour la Santé (DMTS)/SPI/Laboratoire d'Immuno-Allergie Alimentaire, Gif-sur-Yvette, France. * 2 Université Paris-Saclay, INRAE, AgroParisTech, UMR1319 Micalis Institute, Jouy-en-Josas, France. * 3 INRAE, Université Paris-Saclay, BioinfOmics, MIGALE Bioinformatics Facility, Jouy-en-Josas, France. * 4 Université Paris-Saclay, INRAE, MaIAGE, 78350Jouy-en-Josas, France. * 5 Université Paris-Saclay, CEA, INRAE, UMR Département Médicaments et Technologies pour la Santé (DMTS)/SPI/Laboratoire innovations en spectrométrie de masse pour la santé, MetaboHUB, Gif-sur-Yvette, France. * 6 AP-HP, Hôpital Necker-Enfants Malades, Service de Pneumologie et Allergologie Pédiatriques, Paris, France. * 7 Université Paris Cité, Institut Necker Enfants Malades, Equipe Immunorégulation et Immunopathologie, Inserm UMR1151, CNRS, Paris, France. * PMID: **42758777** * DOI: [ 10.1371/journal.pone.0358560 ](https://doi.org/10.1371/journal.pone.0358560) Item in Clipboard Cite Display options Display options Format Abstract PubMed PMID ## Abstract **Background:** Severe asthma is a heterogeneous condition encompassing multiple phenotypes. Understanding lung-specific mechanisms in children with severe asthma may enable the development of more precise therapeutic strategies. We previously reported that immune components in bronchoalveolar lavages (BALs) differentiate children with severe asthma from non-asthmatic disease-controls and, frequent from non-frequent exacerbators, among children with severe asthma. **Objective:** To identify a local signature of severe asthma using complementary multi-omics analyses of BALs. A secondary objective was to evaluate whether bacterial taxa and metabolites discriminate severe asthma subtypes associated with distinct phenotypes or endotypes. **Methods:** BAL microbiome and metabolome were investigated in 20 children with severe asthma and 10 non-asthmatic children using 16S rRNA gene amplicon sequencing and liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS), respectively. Data were analysed separately and through integrative multi-omics approaches. **Results:** Compared with controls, BALs from children with severe asthma showed increased alpha-diversity, higher relative abundances of Actinobacteriota, Streptococcus, Moraxella, Corynebacterium, Tropheryma, and Treponema, and an altered polyamine pathway characterized by reduced arginine and increased spermine and spermidine levels. Integrated analyses revealed significant associations between Streptococcus and both spermine and spermidine. Independently, each dataset discriminated severe asthma phenotypes, notably exacerbation frequency and co-occurring atopic dermatitis. Unsupervised clustering of microbiome profiles identified four distinct clusters that may reflect severe asthma endotypes. **Conclusions:** This study identifies a distinct airway microbiome-metabolome signature associated with pediatric severe asthma. Enrichment of specific bacterial taxa, particularly Streptococcus, together with altered polyamine metabolic pathway, highlights microbial-metabolic interactions potentially involved in disease pathophysiology. The ability of microbiome and metabolome profiles to independently and jointly discriminate clinical phenotypes underscores the relevance of multi-omics approaches for diagnosis and follow-up of sev
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