---
title: "Cell cycle–related biomarkers in sepsis identified by integrated RNA-seq and scRNA-seq"
id: "pubmed-42579704"
canonical_url: "https://medichelpline.com/clinical-feed/pubmed-42579704"
content_type: "clinical_feed_article"
specialty: "Critical Care"
source_name: "PubMed / NCBI"
source_url: "https://pubmed.ncbi.nlm.nih.gov/42579704/"
doi: "10.1371/journal.pone.0355469"
published_at: "2026-08-11T00:00:00.000Z"
evidence_level: "Journal Article"
license: "CC-BY-NC-4.0 / Informational Use"
---
# Cell cycle–related biomarkers in sepsis identified by integrated RNA-seq and scRNA-seq
## Provenance & Clinical Metadata
- **Canonical URL:** https://medichelpline.com/clinical-feed/pubmed-42579704
- **Specialty:** [Critical Care](https://medichelpline.com/clinical-feed/critical-care.md)
- **Primary Source:** PubMed / NCBI
- **Source URL:** [Original Journal Publication](https://pubmed.ncbi.nlm.nih.gov/42579704/)
- **DOI:** [10.1371/journal.pone.0355469](https://doi.org/10.1371%2Fjournal.pone.0355469)
- **Published At:** 2026-08-11T00:00:00.000Z
- **Evidence Rating:** Journal Article
## Executive GIST (TL;DR)
- This study integrated bulk **RNA-seq** and **scRNA-seq** data to identify cell cycle–associated diagnostic biomarkers in **sepsis** using public GEO datasets and machine learning screening. - Differentially expressed genes were overlapped with known **cell cycle–related genes (CCRGs)** to derive candidate biomarkers, which were further filtered by machine learning and validated by expression and ROC analysis. - Four biomarkers—**UPP1**, **DRAM1**, **GADD45A**, and **MAPK14**—were selected and found to be significantly upregulated in sepsis samples with high diagnostic performance (AUC > 0.9). - Gene set enrichment analysis (GSEA) identified 65 pathways shared across all biomarkers, including **toll-like receptor signaling** and **antigen processing and presentation**, linking the biomarkers to immune and pathogen-recognition pathways. - Immune infiltration analysis showed altered proportions of 14 immune cell subsets in sepsis, notably increased **neutrophils** and decreased **CD8+ T cells**. - Drug prediction returned 19 candidate compounds, with **doxorubicin hydrochloride** and **cisplatin** highlighted as having dual-targeting potential against the biomarker-associated network. - Integration of single-cell data annotated six immune cell types and identified **CD16+** and **CD14+ monocytes** as key cells; all four biomarkers showed increasing expression during monocyte differentiation in reconstructed pseudotime trajectories. - The authors conclude these four **cell cycle–associated biomarkers** may link cell-cycle disturbance to sepsis immune dysfunction and warrant further experimental validation and biomarker development. - Conflict of interest: the authors declared no competing interests. Some methodological and cohort details beyond the abstract were not reported in the provided source.
## Clinical Analysis & Structured Key Points
Clipboard, Search History, and several other advanced features are temporarily unavailable. [ Skip to main page content ](https://pubmed.ncbi.nlm.nih.gov/42579704/#article-details) ![U.S. flag](https://cdn.ncbi.nlm.nih.gov/coreutils/uswds/img/favicons/favicon-57.png) An official website of the United States government Here's how you know ![Dot gov](https://cdn.ncbi.nlm.nih.gov/coreutils/uswds/img/icon-dot-gov.svg) **The .gov means it’s official.** Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site. ![Https](https://cdn.ncbi.nlm.nih.gov/coreutils/uswds/img/icon-https.svg) **The site is secure.** The **https://** ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. [ ![NIH NLM Logo](https://cdn.ncbi.nlm.nih.gov/coreutils/nwds/img/logos/AgencyLogo.svg) ](https://www.ncbi.nlm.nih.gov/) [Log in](https://account.ncbi.nlm.nih.gov/?back_url=https%3A%2F%2Fpubmed.ncbi.nlm.nih.gov%2F42579704%2F) Show account info Close #### Account Logged in as: **username** * [Dashboard](https://www.ncbi.nlm.nih.gov/myncbi/) * [Publications](https://www.ncbi.nlm.nih.gov/myncbi/collections/bibliography/) * [Account settings](https://www.ncbi.nlm.nih.gov/account/settings/) * [Log out](https://www.ncbi.nlm.nih.gov/account/signout/?back_url=https%3A//pubmed.ncbi.nlm.nih.gov/42579704/) [Access keys](https://www.ncbi.nlm.nih.gov/guide/browsers/#ncbi_accesskeys) [NCBI Homepage](https://www.ncbi.nlm.nih.gov) [MyNCBI Homepage](https://pubmed.ncbi.nlm.nih.gov/myncbi/) [Main Content](https://pubmed.ncbi.nlm.nih.gov/42579704/#maincontent) [Main Navigation](https://pubmed.ncbi.nlm.nih.gov/42579704/) [ ![pubmed logo](https://cdn.ncbi.nlm.nih.gov/pubmed/af7de7da-df5d-41c6-8de4-8c266af8ccfb/core/images/pubmed-logo-blue.svg) ](https://pubmed.ncbi.nlm.nih.gov/) [ ](https://pubmed.ncbi.nlm.nih.gov/42579704/ "Show search bar") Search: [](https://pubmed.ncbi.nlm.nih.gov/42579704/ "Clear search input")Search [Advanced](https://pubmed.ncbi.nlm.nih.gov/advanced/) [ Clipboard ](https://pubmed.ncbi.nlm.nih.gov/clipboard/) [ User Guide ](https://pubmed.ncbi.nlm.nih.gov/help/) Save Email Send to * [ Clipboard ](https://pubmed.ncbi.nlm.nih.gov/42579704/) * [My Bibliography](https://account.ncbi.nlm.nih.gov/?back_url=https%3A%2F%2Fpubmed.ncbi.nlm.nih.gov%2F42579704%2F%23open-bibliography-panel) * [Collections](https://account.ncbi.nlm.nih.gov/?back_url=https%3A%2F%2Fpubmed.ncbi.nlm.nih.gov%2F42579704%2F%23open-collections-panel) * [Citation manager](https://pubmed.ncbi.nlm.nih.gov/42579704/) Display options Display options Format Abstract PubMed PMID ## Save citation to file Format: Summary (text) PubMed PMID Abstract (text) CSV Create file Cancel ## Email citation Email address has not been verified. Go to [ My NCBI account settings ](https://account.ncbi.nlm.nih.gov/settings/) to confirm your email and then refresh this page. To: Subject: Body: Format: Summary Summary (text) Abstract Abstract (text) MeSH and other data Send email Cancel ### Add to Collections * Create a new collection * Add to an existing collection Name your collection: Name must be less than 100 characters Choose a collection: Unable to load your collection due to an error [Please try again](https://pubmed.ncbi.nlm.nih.gov/42579704/) Add Cancel ### Add to My Bibliography * My Bibliography Unable to load your delegates due to an error [Please try again](https://pubmed.ncbi.nlm.nih.gov/42579704/) Add Cancel ## Your saved search Name of saved search: Search terms: [Test search terms](https://pubmed.ncbi.nlm.nih.gov/42579704/) Would you like email updates of new search results? Saved Search Alert Radio Buttons * Yes * No Email: ([change](https://www.ncbi.nlm.nih.gov/account/settings/)) Frequency: Monthly Weekly Daily Which day? The first Sunday The first Monday The first Tuesday The first Wednesday The first Thursday The first Friday The first Saturday The first day The first weekday Which day? Sunday Monday Tuesday Wednesday Thursday Friday Saturday Report format: Summary Summary (text) Abstract Abstract (text) PubMed Send at most: 1 item 5 items 10 items 20 items 50 items 100 items 200 items Send even when there aren't any new results Optional text in email: Save Cancel ## Create a file for external citation management software Create file Cancel ## Your RSS Feed Name of RSS Feed: Number of items displayed: 5 10 15 20 50 100 Create RSS Cancel RSS Link Copy ### Actions Cite Collections Add to Collections * Create a new collection * Add to an existing collection Name your collection: Name must be less than 100 characters Choose a collection: Unable to load your collection due to an error [Please try again](https://pubmed.ncbi.nlm.nih.gov/42579704/) Add Cancel Permalink Permalink Copy Display options Display options Format Abstract PubMed PMID ### Page navigation * [ Title & authors ](https://pubmed.ncbi.nlm.nih.gov/42579704/#heading) * [ Abstract ](https://pubmed.ncbi.nlm.nih.gov/42579704/#abstract) * [ Conflict of interest statement ](https://pubmed.ncbi.nlm.nih.gov/42579704/#conflict-of-interest) * [ MeSH terms ](https://pubmed.ncbi.nlm.nih.gov/42579704/#mesh-terms) * [ Substances ](https://pubmed.ncbi.nlm.nih.gov/42579704/#substances) Title & authors Abstract Conflict of interest statement MeSH terms Substances PLoS One Actions * [ Search in PubMed ](https://pubmed.ncbi.nlm.nih.gov/?term=%22PLoS+One%22%5Bjour%5D&sort=date&sort_order=desc) * [ Search in NLM Catalog ](https://www.ncbi.nlm.nih.gov/nlmcatalog?term=%22PLoS+One%22%5BTitle+Abbreviation%5D) * [ Add to Search ](https://pubmed.ncbi.nlm.nih.gov/42579704/) . 2026 Aug 11;21(8):e0355469. doi: 10.1371/journal.pone.0355469. eCollection 2026. # Integration of RNA-seq and scRNA-seq to investigate the role of cell cycle-related biomarkers in sepsis [Mei-Ping Zheng](https://pubmed.ncbi.nlm.nih.gov/?term=Zheng+MP&cauthor_id=42579704)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42579704/#full-view-affiliation-1 "Department of Anesthesiology, Sanming First Hospital Affiliated to Fujian Medical University, Sanming, China."), [Yan-Ling Du](https://pubmed.ncbi.nlm.nih.gov/?term=Du+YL&cauthor_id=42579704)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42579704/#full-view-affiliation-1 "Department of Anesthesiology, Sanming First Hospital Affiliated to Fujian Medical University, Sanming, China."), [Xiong-Bin Liao](https://pubmed.ncbi.nlm.nih.gov/?term=Liao+XB&cauthor_id=42579704)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42579704/#full-view-affiliation-1 "Department of Anesthesiology, Sanming First Hospital Affiliated to Fujian Medical University, Sanming, China."), [Ming-Quan Qiu](https://pubmed.ncbi.nlm.nih.gov/?term=Qiu+MQ&cauthor_id=42579704)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42579704/#full-view-affiliation-1 "Department of Anesthesiology, Sanming First Hospital Affiliated to Fujian Medical University, Sanming, China."), [Huatian Luo](https://pubmed.ncbi.nlm.nih.gov/?term=Luo+H&cauthor_id=42579704)[ 2 ](https://pubmed.ncbi.nlm.nih.gov/42579704/#full-view-affiliation-2 "Department of Breast Surgery, Sanming First Hospital Affiliated to Fujian Medical University, Sanming, China."), [Xiao-Tan Gao](https://pubmed.ncbi.nlm.nih.gov/?term=Gao+XT&cauthor_id=42579704)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42579704/#full-view-affiliation-1 "Department of Anesthesiology, Sanming First Hospital Affiliated to Fujian Medical University, Sanming, China.") Affiliations Expand ### Affiliations * 1 Department of Anesthesiology, Sanming First Hospital Affiliated to Fujian Medical University, Sanming, China. * 2 Department of Breast Surgery, Sanming First Hospital Affiliated to Fujian Medical University, Sanming, China. * PMID: **42579704** * DOI: [ 10.1371/journal.pone.0355469 ](https://doi.org/10.1371/journal.pone.0355469) Item in Clipboard # Integration of RNA-seq and scRNA-seq to investigate the role of cell cycle-related biomarkers in sepsis Mei-Ping Zheng et al. PLoS One. 2026. Show details Display options Display options Format Abstract PubMed PMID PLoS One Actions * [ Search in PubMed ](https://pubmed.ncbi.nlm.nih.gov/?term=%22PLoS+One%22%5Bjour%5D&sort=date&sort_order=desc) * [ Search in NLM Catalog ](https://www.ncbi.nlm.nih.gov/nlmcatalog?term=%22PLoS+One%22%5BTitle+Abbreviation%5D) * [ Add to Search ](https://pubmed.ncbi.nlm.nih.gov/42579704/) . 2026 Aug 11;21(8):e0355469. doi: 10.1371/journal.pone.0355469. eCollection 2026. ### Authors [Mei-Ping Zheng](https://pubmed.ncbi.nlm.nih.gov/?term=Zheng+MP&cauthor_id=42579704)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42579704/#short-view-affiliation-1 "Department of Anesthesiology, Sanming First Hospital Affiliated to Fujian Medical University, Sanming, China."), [Yan-Ling Du](https://pubmed.ncbi.nlm.nih.gov/?term=Du+YL&cauthor_id=42579704)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42579704/#short-view-affiliation-1 "Department of Anesthesiology, Sanming First Hospital Affiliated to Fujian Medical University, Sanming, China."), [Xiong-Bin Liao](https://pubmed.ncbi.nlm.nih.gov/?term=Liao+XB&cauthor_id=42579704)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42579704/#short-view-affiliation-1 "Department of Anesthesiology, Sanming First Hospital Affiliated to Fujian Medical University, Sanming, China."), [Ming-Quan Qiu](https://pubmed.ncbi.nlm.nih.gov/?term=Qiu+MQ&cauthor_id=42579704)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42579704/#short-view-affiliation-1 "Department of Anesthesiology, Sanming First Hospital Affiliated to Fujian Medical University, Sanming, China."), [Huatian Luo](https://pubmed.ncbi.nlm.nih.gov/?term=Luo+H&cauthor_id=42579704)[ 2 ](https://pubmed.ncbi.nlm.nih.gov/42579704/#short-view-affiliation-2 "Department of Breast Surgery, Sanming First Hospital Affiliated to Fujian Medical University, Sanming, China."), [Xiao-Tan Gao](https://pubmed.ncbi.nlm.nih.gov/?term=Gao+XT&cauthor_id=42579704)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42579704/#short-view-affiliation-1 "Department of Anesthesiology, Sanming First Hospital Affiliated to Fujian Medical University, Sanming, China.") ### Affiliations * 1 Department of Anesthesiology, Sanming First Hospital Affiliated to Fujian Medical University, Sanming, China. * 2 Department of Breast Surgery, Sanming First Hospital Affiliated to Fujian Medical University, Sanming, China. * PMID: **42579704** * DOI: [ 10.1371/journal.pone.0355469 ](https://doi.org/10.1371/journal.pone.0355469) Item in Clipboard Cite Display options Display options Format Abstract PubMed PMID ## Abstract **Background:** Sepsis is a life-threatening organ dysfunction arising from a dysregulated host response to infection. Cell-cycle disturbance is increasingly recognized as a driver of sepsis-associated immune dysfunction. This study aimed to identify cell cycle-associated diagnostic biomarkers and clarify their roles in sepsis. **Methods:** Transcriptomic profiles from the Gene Expression Omnibus (GEO) database were analyzed to identify candidate genes by overlapping differentially expressed genes (DEGs) between sepsis and control samples with cell cycle-related genes (CCRGs). Biomarkers were subsequently screened via machine learning algorithms, followed by expression level validation and receiver operating characteristic (ROC) curve analysis. Furthermore, gene set enrichment analysis (GSEA), immune infiltration analysis, and drug prediction were performed.Finally, single-cell RNA sequencing (scRNA-seq) data were integrated for cell annotation and biomarker expression analysis, enabling the identification of key cells and the reconstruction of pseudotime trajectories. **Results:** UPP1, DRAM1, GADD45A, and MAPK14 were selected as biomarkers and were significantly upregulated in sepsis samples (area under the curve (AUC) > 0.9). Additionally, GSEA revealed 65 pathways that were shared across all biomarkers, such as toll-like receptor signaling and antigen processing and presentation. Immune analysis revealed altered infiltration of 14 cell subsets in sepsis, including increased neutrophil numbers and decreased CD8+ T cell numbers. Drug prediction analysis identified 19 potential drugs, including doxorubicin hydrochloride and cisplatin with dual-targeting capacity. Finally, scRNA-seq confirmed CD16+ and CD14+ monocytes as key cells among the six cell types, with all biomarkers showing increasing expression trends during their differentiation. **Conclusion:** This study identified four cell cycle-associated biomarkers for sepsis and provided computational evidence linking them to sepsis-related pathways and monocyte differentiation. These findings may provide useful clues for future experimental validation and biomarker development. Copyright: © 2026 Zheng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. [PubMed Disclaimer](https://pubmed.ncbi.nlm.nih.gov/disclaimer/) ## Conflict of interest statement The authors have declared that no competing interests exist. ## MeSH terms * Biomarkers / metabolism Actions * [ Search in PubMed ](https://pubmed.ncbi.nlm.nih.gov/?term=%22Biomarkers%2Fmetabolism%22%5BMeSH%5D&sort=date&sort_order=desc) * [ Search in MeSH ](https://www.ncbi.nlm.nih.gov/mesh?term=Biomarkers) * [ Add to Search ](https://pubmed.ncbi.nlm.nih.gov/42579704/) * Cell Cycle Proteins / genetics Actions * [ Search in PubMed ](https://pubmed.ncbi.nlm.nih.gov/?term=%22Cell+Cycle+Proteins%2Fgenetics%22%5BMeSH%5D&sort=date&sort_order=desc) * [ Search in MeSH ](https://www.ncbi.nlm.nih.gov/mesh?term=Cell+Cycle+Proteins) * [ Add to Search ](https://pubmed.ncbi.nlm.nih.gov/42579704/) * Cell Cycle* / genetics Actions * [ Search in PubMed ](https://pubmed.ncbi.nlm.nih.gov/?term=%22Cell+Cycle%2Fgenetics%22%5BMAJR%5D&sort=date&sort_order=desc) * [ Search in MeSH ](https://www.ncbi.nlm.nih.gov/mesh?term=Cell+Cycle) * [ Add to Search ](https://pubmed.ncbi.nlm.nih.gov/42579704/) * Gene Expression Profiling Actions * [ Search in PubMed ](https://pubmed.ncbi.nlm.nih.gov/?term=%22Gene+Expression+Profiling%22%5BMeSH%5D&sort=date&sort_order=desc) * [ Search in MeSH ](https://www.ncbi.nlm.nih.gov/mesh?term=Gene+Expression+Profiling) * [ Add to Search ](https://pubmed.ncbi.nlm.nih.gov/42579704/) * Humans Actions * [ Search in PubMed ](https://pubmed.ncbi.nlm.nih.gov/?term=%22Humans%22%5BMeSH%5D&sort=date&sort_order=desc) * [ Search in MeSH ](https://www.ncbi.nlm.nih.gov/mesh?term=Humans) * [ Add to Search ](https://pubmed.ncbi.nlm.nih.gov/42579704/) * RNA-Seq* / methods Actions * [ Search in PubMed ](https://pubmed.ncbi.nlm.nih.gov/?term=%22RNA-Seq%2Fmethods%22%5BMAJR%5D&sort=date&sort_order=desc) * [ Search in MeSH ](https://www.ncbi.nlm.nih.gov/mesh?term=RNA-Seq) * [ Add to Search ](https://pubmed.ncbi.nlm.nih.gov/42579704/) * Sepsis* / genetics Actions * [ Search in PubMed ](https://pubmed.ncbi.nlm.nih.gov/?term=%22Sepsis%2Fgenetics%22%5BMAJR%5D&sort=date&sort_order=desc) * [ Search in MeSH ](https://www.ncbi.nlm.nih.gov/mesh?term=Sepsis) * [ Add to Search ](https://pubmed.ncbi.nlm.nih.gov/42579704/) * Sepsis* / metabolism Actions * [ Search in PubMed ](https://pubmed.ncbi.nlm.nih.gov/?term=%22Sepsis%2Fmetabolism%22%5BMAJR%5D&sort=date&sort_order=desc) * [ Search in MeSH ](https://www.ncbi.nlm.nih.gov/mesh?term=Sepsis) * [ Add to Search ](https://pubmed.ncbi.nlm.nih.gov/42579704/) * Sepsis* / pathology Actions * [ Search in PubMed ](https://pubmed.ncbi.nlm.nih.gov/?term=%22Sepsis%2Fpathology%22%5BMAJR%5D&sort=date&sort_order=desc) * [ Search in MeSH ](https://www.ncbi.nlm.nih.gov/mesh?term=Sepsis) * [ Add to Search ](https://pubmed.ncbi.nlm.nih.gov/42579704/) * Single-Cell Analysis Actions * [ Search in PubMed ](https://pubmed.ncbi.nlm.nih.gov/?term=%22Single-Cell+Analysis%22%5BMeSH%5D&sort=date&sort_order=desc) * [ Search in MeSH ](https://www.ncbi.nlm.nih.gov/mesh?term=Single-Cell+Analysis) * [ Add to Search ](https://pubmed.ncbi.nlm.nih.gov/42579704/) * Single-Cell Gene Expression Analysis Actions * [ Search in PubMed ](https://pubmed.ncbi.nlm.nih.gov/?term=%22Single-Cell+Gene+Expression+Analysis%22%5BMeSH%5D&sort=date&sort_order=desc) * [ Search in MeSH ](https://www.ncbi.nlm.nih.gov/mesh?term=Single-Cell+Gene+Expression+Analysis) * [ Add to Search ](https://pubmed.ncbi.nlm.nih.gov/42579704/) * Transcriptome Actions * [ Search in PubMed ](https://pubmed.ncbi.nlm.nih.gov/?term=%22Transcriptome%22%5BMeSH%5D&sort=date&sort_order=desc) * [ Search in MeSH ](https://www.ncbi.nlm.nih.gov/mesh?term=Transcriptome) * [ Add to Search ](https://pubmed.ncbi.nlm.nih.gov/42579704/) ## Substances * Biomarkers Actions * [ Search in PubMed ](https://pubmed.ncbi.nlm.nih.gov/?term=%22Biomarkers%22%5Bnm%5D&sort=date&sort_order=desc) * [ Search in MeSH ](https://www.ncbi.nlm.nih.gov/mesh?term=%22Biomarkers%22) * [ Add to Search ](https://pubmed.ncbi.nlm.nih.gov/42579704/) * Cell Cycle Proteins Actions * [ Search in PubMed ](https://pubmed.ncbi.nlm.nih.gov/?term=%22Cell+Cycle+Proteins%22%5Bnm%5D&sort=date&sort_order=desc) * [ Search in MeSH ](https://www.ncbi.nlm.nih.gov/mesh?term=%22Cell+Cycle+Proteins%22) * [ Add to Search ](https://pubmed.ncbi.nlm.nih.gov/42579704/) [x] Cite Copy Download .nbib .nbib Format: AMA APA MLA NLM **Send To** * [Clipboard](https://pubmed.ncbi.nlm.nih.gov/42579704/) * [Email](https://account.ncbi.nlm.nih.gov/?back_url=https%3A%2F%2Fpubmed.ncbi.nlm.nih.gov%2F42579704%2F%23open-email-panel) * [Save](https://pubmed.ncbi.nlm.nih.gov/42579704/) * [My Bibliography](https://account.ncbi.nlm.nih.gov/?back_url=https%3A%2F%2Fpubmed.ncbi.nlm.nih.gov%2F42579704%2F%23open-bibliography-panel) * [Collections](https://account.ncbi.nlm.nih.gov/?back_url=https%3A%2F%2Fpubmed.ncbi.nlm.nih.gov%2F42579704%2F%23open-collections-panel) * [Citation Manager](https://pubmed.ncbi.nlm.nih.gov/42579704/) [x] NCBI Literature Resources [MeSH](https://www.ncbi.nlm.nih.gov/mesh/) [PMC](https://www.ncbi.nlm.nih.gov/pmc/) [Bookshelf](https://www.ncbi.nlm.nih.gov/books) [Disclaimer](https://pubmed.ncbi.nlm.nih.gov/disclaimer/) The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited. Follow NCBI [ Twitter ](https://twitter.com/ncbi) [ Facebook ](https://www.facebook.com/ncbi.nlm) [ LinkedIn ](https://www.linkedin.com/company/ncbinlm) [ GitHub ](https://github.com/ncbi) [ ](https://ncbiinsights.ncbi.nlm.nih.gov/) [Connect with NLM](https://www.nlm.nih.gov/socialmedia/index.html) * [ Twitter ](https://twitter.com/NLM_NIH) * [ SM-Facebook ](https://www.facebook.com/nationallibraryofmedicine) * [ SM-Youtube ](https://www.youtube.com/user/NLMNIH) National Library of Medicine [8600 Rockville Pike Bethesda, MD 20894](https://www.google.com/maps/place/8600+Rockville+Pike,+Bethesda,+MD+20894/@38.9959508,-77.101021,17z/data=!3m1!4b1!4m5!3m4!1s0x89b7c95e25765ddb:0x19156f88b27635b8!8m2!3d38.9959508!4d-77.0988323) [Web Policies](https://www.nlm.nih.gov/web_policies.html) [FOIA](https://www.nih.gov/institutes-nih/nih-office-director/office-communications-public-liaison/freedom-information-act-office) [HHS Vulnerability Disclosure](https://www.hhs.gov/vulnerability-disclosure-policy/index.html) [Help](https://support.nlm.nih.gov/?pagename=pubmed%3Apubmed%3Aabstract%3ANONE) [Accessibility](https://www.nlm.nih.gov/accessibility.html) [Careers](https://www.nlm.nih.gov/c
## Related Clinical Research

- [Response-Adapted Bladder Preservation in MIBC: RETAIN-2 Phase II Results and ctDNA Dynamics](https://medichelpline.com/clinical-feed/medrxiv-2-response-adapted-bladder-preservation-in-muscle-invasive-bladder-cancer-results.md)
- [Modified SIOPEL-4 Shows Superior Outcomes in High-Risk Pediatric Hepatoblastoma](https://medichelpline.com/clinical-feed/pubmed-42595556.md) (DOI: 10.3760/cma.j.cn112152-20251121-00580)
- [Gene-based immunotherapy in osteosarcoma: full article text not available on source page](https://medichelpline.com/clinical-feed/frontiers-in-immunology-16-gene-based-immunotherapy-in-osteosarcoma-from-oncolytic-vectors-to-engineered.md)
- [Icaritin Combined with TACE for Hepatocellular Carcinoma: Analysis by Child‑Pugh Class](https://medichelpline.com/clinical-feed/frontiers-in-immunology-12-efficacy-analysis-of-icaritin-combined-with-transarterial-chemoembolization-in.md)
- [TRPV1-targeted Fe-phenolate (FeOLDA) induces apoptosis and ferroptosis in NSCLC](https://medichelpline.com/clinical-feed/pubmed-42411334.md) (DOI: 10.1039/d6bm00434b)

## Navigation
- [← Back to Critical Care Feed](https://medichelpline.com/clinical-feed/critical-care.md)
- [← All Clinical Specialties](https://medichelpline.com/clinical-feed.md)
## Medical & Regulatory Disclaimer

> [!CAUTION]
> MedicHelpline content is structured for research, educational, and professional discovery purposes. It does not constitute individual medical advice, clinical diagnosis, or treatment recommendations.
> Always verify dosing, contraindications, and regulatory alerts against official product labeling and primary regulatory sources before clinical decision-making.