---
title: "Serum IL-6, IL-10 and IL6/IL10 Genotypes in Bangladeshi Patients with Diabetic Neuropathy"
id: "plos-one-14-association-of-il-6-and-il-10-serum-levels-with-genetic-variants-in-diabetic"
canonical_url: "https://medichelpline.com/clinical-feed/plos-one-14-association-of-il-6-and-il-10-serum-levels-with-genetic-variants-in-diabetic"
content_type: "clinical_feed_article"
specialty: "Endocrinology"
source_name: "PLOS ONE (Medicine)"
source_url: "https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0360933"
published_at: "2026-10-22T14:00:00.000Z"
evidence_level: "Journal Feed"
license: "CC-BY-NC-4.0 / Informational Use"
---
# Serum IL-6, IL-10 and IL6/IL10 Genotypes in Bangladeshi Patients with Diabetic Neuropathy
## Provenance & Clinical Metadata
- **Canonical URL:** https://medichelpline.com/clinical-feed/plos-one-14-association-of-il-6-and-il-10-serum-levels-with-genetic-variants-in-diabetic
- **Specialty:** [Endocrinology](https://medichelpline.com/clinical-feed/endocrinology.md)
- **Primary Source:** PLOS ONE (Medicine)
- **Source URL:** [Original Journal Publication](https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0360933)
- **Published At:** 2026-10-22T14:00:00.000Z
- **Evidence Rating:** Journal Feed
## Executive GIST (TL;DR)
- A case-control study enrolled 225 participants divided equally into three groups: T2DM with diabetic neuropathy (DN), T2DM without DN, and healthy controls. - Investigators measured serum levels of **IL-6** and **IL-10** by ELISA and genotyped IL6 (rs1800796) and IL10 (rs1800896) using PCR-based methods. - DN diagnosis combined clinical examination, 10 g monofilament, 128 Hz tuning fork, pinprick testing and the Michigan Neuropathy Screening Instrument (MNSI ≥2.5). - Serum **IL-6** concentrations were significantly higher in the DN group than in both diabetic and healthy comparator groups (p < 0.0001), with highest levels among carriers of the IL6 rs1800796 CC genotype. - Serum **IL-10** concentrations did not differ significantly across groups (p = 0.0948); genotype-phenotype associations for IL-10 were not robust in adjusted models. - In unadjusted analyses, the IL6 CC genotype and IL10 GG genotype were associated with increased odds of DN versus healthy controls, but neither genotype remained independently associated with DN after multivariable adjustment. - Combined genotype analyses produced several exploratory, nominal associations; authors described these as preliminary and not definitive. - The study team reported that serum **IL-6** demonstrated discriminatory ability to distinguish DN cases from comparators, whereas **IL-10** performed poorly as a classifier. - Investigators noted limitations including sample size, case-control design, single‑population recruitment (predominantly Bengali), and the need for larger prospective cohorts with external validation before clinical application.
## Clinical Analysis & Structured Key Points
Association of IL-6 and IL-10 serum levels with genetic variants in diabetic neuropathy: Clinical findings from a Bangladeshi patient cohort | PLOS One Browse Subject Areas ? Click through the PLOS taxonomy to find articles in your field. For more information about PLOS Subject Areas, click here . Article Authors Metrics Comments Media Coverage Peer Review Reader Comments Figures Figures Abstract Background Diabetic Neuropathy (DN) is a prevalent complication of diabetes linked to chronic inflammation. Interleukin-6 (IL-6) and Interleukin-10 (IL-10) are key cytokines implicated in DN pathogenesis. This study investigates the association of IL6 and IL10 gene polymorphisms and serum levels with DN in the Bangladeshi population. Materials and methods A case-control study was conducted with 225 participants: diabetic patients with DN (n = 75), diabetic patients without DN (n = 75), and healthy controls (n = 75). Genomic DNA was extracted for genotyping IL-6 (rs1800796) and IL-10 (rs1800896) SNPs via PCR. Serum cytokine levels were measured using ELISA. Results Serum IL-6 levels were significantly higher in the DN group than in the other study groups (p 1 indicated increased odds, whereas an OR 0.05 indicated no significant deviation from HWE. Associations between continuous variables were evaluated using Pearson’s correlation coefficient when assumptions of approximate normality, linearity, and absence of influential outliers were satisfied; otherwise, Spearman’s rank correlation coefficient was used. The discriminatory performance of serum IL-6 and IL-10 concentrations for diabetic neuropathy was explored using receiver operating characteristic (ROC) curve analysis. The area under the curve (AUC) with 95% CIs was calculated, and exploratory optimal cut-off values were determined using the maximum Youden index. Corresponding sensitivity and specificity values were also reported. In the tables, p < 0.05 and p < 0.01 indicate nominal statistical significance. As no formal adjustment for multiple comparisons was applied, the results of exploratory genetic and subgroup analyses were interpreted cautiously. Results Socio-demographic, anthropometric, and biochemical characteristics of participants The study assessed socio-demographic, anthropometric, and biochemical profiles of three groups: T2DM patients with diabetic neuropathy (DM with DN), T2DM patients without neuropathy (DM without DN), and healthy controls (HC). The DM with DN group had a significantly higher mean age (62.64 ± 0.8455 years) than the DM without DN (54.07 ± 0.9809) and healthy control groups (48.55 ± 1.226) (p < 0.0001), while gender distribution remained balanced across all groups. Urban residency was more prevalent in the DM with DN group (77.33%) compared to the DM without DN (28.00%) and controls (44.00%) (p < 0.0001). Anthropometric measurements showed significantly higher body weight and BMI in the DM with DN group (70.19 ± 1.14 kg, 25.19 ± 0.32 kg/m 2 ) compared to other groups (p < 0.0001). The average age of diabetes onset was higher in the DN group (50.83 ± 0.73 years; p = 0.0007), with an average DN duration of 8.33 ± 0.25 years. Both systolic and diastolic blood pressures were significantly elevated in the DM with DN group (p < 0.0001). Biochemically, fasting blood sugar, random blood sugar, and HbA1c levels were significantly higher in the DM with DN group (p < 0.0001). Lipid profiles revealed elevated total cholesterol, triglycerides, and LDL, along with lower HDL in the DN group (p < 0.01). Renal function markers (BUN and creatinine) and inflammatory marker CRP were also significantly increased (p < 0.01), as were liver enzymes AST and ALT (p < 0.01), indicating greater systemic metabolic disturbance in the DM with DN group ( Table 2 ). Download: PNG larger image TIFF original image Table 2. Socio-demographic, anthropometric, and biochemical characteristics of study participants. https://doi.org/10.1371/journal.pone.0360933.t002 Genotypic analysis of IL-6 and IL-10 polymorphisms The T-ARMS was employed for genotyping the IL-6 rs180079
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