---
title: "Agave Rubisco activase complements Setaria mutant and partially restores photosynthesis and growth"
id: "biorxiv-0-complementation-of-a-setaria-rubisco-activase-mutant-with-agave-rubisco"
canonical_url: "https://medichelpline.com/clinical-feed/biorxiv-0-complementation-of-a-setaria-rubisco-activase-mutant-with-agave-rubisco"
content_type: "clinical_feed_article"
specialty: "General"
source_name: "bioRxiv (Biomedical Preprints)"
source_url: "https://www.biorxiv.org/content/10.64898/2026.08.28.747894v1?rss=1"
published_at: "2026-09-03T12:00:00.000Z"
evidence_level: "Verified Feed"
license: "CC-BY-NC-4.0 / Informational Use"
---
# Agave Rubisco activase complements Setaria mutant and partially restores photosynthesis and growth
## Provenance & Clinical Metadata
- **Canonical URL:** https://medichelpline.com/clinical-feed/biorxiv-0-complementation-of-a-setaria-rubisco-activase-mutant-with-agave-rubisco
- **Specialty:** [General](https://medichelpline.com/clinical-feed/general.md)
- **Primary Source:** bioRxiv (Biomedical Preprints)
- **Source URL:** [Original Journal Publication](https://www.biorxiv.org/content/10.64898/2026.08.28.747894v1?rss=1)
- **Published At:** 2026-09-03T12:00:00.000Z
- **Evidence Rating:** Verified Feed
## Executive GIST (TL;DR)
- The study tested whether Rubisco activase (RCA) from the CAM plant Agave tequilana (AtRCA) can functionally replace endogenous RCA in the C4 model Setaria viridis. - Authors generated transgenic Setaria lines expressing AtRCA and a complementation line where the native SvRCAβ null mutant (ΔrcaB) was rescued with AtRCA (AtRCA[bKO]). - In vitro assays showed **Agave RCA** activates **Setaria Rubisco** and exhibits greater thermostability than Setaria RCA. - AtRCA[bKO] plants exhibited restored growth relative to the mutant background, indicating functional complementation by AtRCA. - At 25°C, AtRCA[bKO] plants had higher **CO2 assimilation** rates than wild-type (WT) and AtRCA transgenic lines, despite no detectable changes in Rubisco content or activation state. - After six hours of heat stress, AtRCA[bKO] plants retained higher CO2 assimilation than the AtRCA transgenic line, but after 24 hours CO2 assimilation declined to similar levels across all lines. - The data suggest AtRCA improves short-term photosynthetic performance and has higher thermostability, but RCA may not be the sole or limiting factor for carbon assimilation under the tested heat conditions. - The authors conclude that substitution of RCA from a CAM species into a C4 model supports normal growth and can confer modest increases in carbon assimilation under control and short-term heat stress. - This report is a preprint and has not undergone peer review; experimental details beyond those summarized in the abstract were not reported in the source provided.
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Amber Hotto Boyce Thompson Institute * [Find this author on Google Scholar](https://www.biorxiv.org/lookup/google-scholar?link_type=googlescholar&gs_type=author&author%5B0%5D=Amber%2BHotto%2B "Open in new tab") * [Find this author on PubMed](https://www.biorxiv.org/lookup/external-ref?access_num=Hotto%20A&link_type=AUTHORSEARCH "Open in new tab") * [Search for this author on this site](https://www.biorxiv.org/search/author1%3AAmber%2BHotto%2B) Sarah Gartner Boyce Thompson Institute * [Find this author on Google Scholar](https://www.biorxiv.org/lookup/google-scholar?link_type=googlescholar&gs_type=author&author%5B0%5D=Sarah%2BGartner%2B "Open in new tab") * [Find this author on PubMed](https://www.biorxiv.org/lookup/external-ref?access_num=Gartner%20S&link_type=AUTHORSEARCH "Open in new tab") * [Search for this author on this site](https://www.biorxiv.org/search/author1%3ASarah%2BGartner%2B) Kathryn Eshenour Boyce Thompson Institute * [Find this author on Google Scholar](https://www.biorxiv.org/lookup/google-scholar?link_type=googlescholar&gs_type=author&author%5B0%5D=Kathryn%2BEshenour%2B "Open in new tab") * [Find this author on PubMed](https://www.biorxiv.org/lookup/external-ref?access_num=Eshenour%20K&link_type=AUTHORSEARCH "Open in new tab") * [Search for this author on this site](https://www.biorxiv.org/search/author1%3AKathryn%2BEshenour%2B) David B Stern Boyce Thompson Institute * [Find this author on Google Scholar](https://www.biorxiv.org/lookup/google-scholar?link_type=googlescholar&gs_type=author&author%5B0%5D=David%2BB%2BStern%2B "Open in new tab") * [Find this author on PubMed](https://www.biorxiv.org/lookup/external-ref?access_num=Stern%20DB&link_type=AUTHORSEARCH "Open in new tab") * [Search for this author on this site](https://www.biorxiv.org/search/author1%3ADavid%2BB%2BStern%2B) * [ORCID record for David B Stern](http://orcid.org/0000-0002-0653-6602 "Open in new tab") * For correspondence: ds28@cornell.edu * [Abstract](https://www.biorxiv.org/content/10.64898/2026.08.28.747894v1)[](https://www.biorxiv.org/panels_ajax_tab/biorxiv_tab_art/node:5754401/1) * [Info/History](https://www.biorxiv.org/content/10.64898/2026.08.28.747894v1.article-info)[](https://www.biorxiv.org/panels_ajax_tab/biorxiv_tab_info/node:5754401/1) * [Metrics](https://www.biorxiv.org/content/10.64898/2026.08.28.747894v1.article-metrics)[](https://www.biorxiv.org/panels_ajax_tab/article_tab_metrics/node:5754401/1) * [ Preview PDF](https://www.biorxiv.org/content/10.64898/2026.08.28.747894v1.full.pdf+html)[](https://www.biorxiv.org/panels_ajax_tab/biorxiv_tab_pdf/node:5754401/1) ![Loading](https://www.biorxiv.org/sites/all/modules/contrib/panels_ajax_tab/images/loading.gif) ## Abstract Photosynthetic carbon assimilation is sensitive to heat stress, with a key component being the thermal sensitivity of Rubisco activase (RCA). One approach to increasing heat tolerance of photosynthesis is to increase the thermotolerance of RCA. Here, we have used a transgenic approach to express RCAβ from Agave tequilana (AtRCA) in Setaria viridis. A second line was created where the endogenous SvRCAβ was substituted for AtRCAβ through complementation of a null mutant, ∆rcaB (AtRCA[bKO]). In vitro assays showed that Agave RCA readily activates Setaria Rubisco, and that its thermostability is higher than that of Setaria RCA. AtRCA[bKO] plants exhibited higher CO2 assimilation at 25°C compared to WT and AtRCA plants, although Rubisco content and activation did not change. After six hours of heat stress, AtRCA[bKO] plants retained higher CO2 assimilation rates than AtRCA, however, after 24 hours CO2 assimilation declined to similar levels in all lines, suggesting that RCA may not limit carbon assimilation at the conditions tested. Taken together, our results show that substitution of RCA from a CAM plant into a C4 model allows normal growth and supports slightly increased carbon assimilation under control and short-term heat conditions. ### Competing Interest Statement The authors have declared no competing interest. Copyright The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission. bioRxiv and medRxiv thank the following for their generous financial support: > The Chan Zuckerberg Initiative, Cold Spring Harbor Laboratory, the Sergey Brin Family Foundation, California Institute of Technology, Centre National de la Recherche Scientifique, Fred Hutchinson Cancer Center, Imperial College London, Massachusetts Institute of Technology, Stanford University, The University of Edinburgh, University of Washington, and Vrije Universiteit Amsterdam. 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[ Download PDF](https://www.biorxiv.org/content/10.64898/2026.08.28.747894v1.full.pdf) Print/Save Options [Download PDF](https://www.biorxiv.org/content/biorxiv/early/2026/09/02/2026.08.28.747894.full.pdf)Full Text & In-line FiguresXML [More Info](https://www.biorxiv.org/about/FAQ#PrintOptions "More Information on Print/Save Options") [ Email](https://www.biorxiv.org/ "Email this Article") [ Share](https://www.biorxiv.org/) Complementation of a Setaria Rubisco activase mutant with Agave Rubisco activase restores growth and photosynthesis Amber Hotto, Sarah Gartner, Kathryn Eshenour, David B Stern bioRxiv 2026.08.28.747894; doi: https://doi.org/10.64898/2026.08.28.747894 This article is a preprint and has not been certified by peer review [[what does this mean?](https://www.biorxiv.org/about/FAQ#unrefereed)]. 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