---
title: "Kdm5 and ARGONAUTE proteins Piwi and Aubergine control female abdominal pigmentation in Drosophila"
id: "biorxiv-0-the-histone-demethylase-kdm5-and-the-argonaute-proteins-piwi-and-aubergine"
canonical_url: "https://medichelpline.com/clinical-feed/biorxiv-0-the-histone-demethylase-kdm5-and-the-argonaute-proteins-piwi-and-aubergine"
content_type: "clinical_feed_article"
specialty: "General"
source_name: "bioRxiv (Biomedical Preprints)"
source_url: "https://www.biorxiv.org/content/10.64898/2026.08.27.747613v1?rss=1"
published_at: "2026-09-03T11:54:36.000Z"
evidence_level: "Verified Feed"
license: "CC-BY-NC-4.0 / Informational Use"
---
# Kdm5 and ARGONAUTE proteins Piwi and Aubergine control female abdominal pigmentation in Drosophila
## Provenance & Clinical Metadata
- **Canonical URL:** https://medichelpline.com/clinical-feed/biorxiv-0-the-histone-demethylase-kdm5-and-the-argonaute-proteins-piwi-and-aubergine
- **Specialty:** [General](https://medichelpline.com/clinical-feed/general.md)
- **Primary Source:** bioRxiv (Biomedical Preprints)
- **Source URL:** [Original Journal Publication](https://www.biorxiv.org/content/10.64898/2026.08.27.747613v1?rss=1)
- **Published At:** 2026-09-03T11:54:36.000Z
- **Evidence Rating:** Verified Feed
## Executive GIST (TL;DR)
- In Drosophila melanogaster, **female abdominal pigmentation** is sexually dimorphic: males show fully pigmented posterior segments while females display a posterior melanin stripe. Pigmentation depends on regulated expression of pigment-synthesis enzyme genes during pupal and young adult stages. - An RNAi screen driven by the pupal epidermis driver yellow-Gal4 identified the histone demethylase **Kdm5** as required for normal female abdominal pigmentation; Kdm5 removes the **H3K4me3** histone mark placed by the methyltransferase **Trithorax (Trx)**. - Down-regulation of **Kdm5** reduces abdominal pigmentation and phenocopies **trx** down-regulation, indicating they act in the same regulatory axis for pigment production. - Kdm5 promotes melanin production at least in part by regulating the pigmentation gene **tan**. - Transcriptomic analysis of pupal abdominal epidermis showed substantial overlap between **Kdm5** and **Trx** targets, including components of the **piRNA** pathway such as **piwi** and **aubergine**. - The ARGONAUTE proteins **Piwi** and **Aubergine**, traditionally linked to germline transposon silencing, also have somatic functions in several tissues; this study demonstrates they participate in establishing female abdominal pigmentation. - Evidence suggests **Piwi** and **Aubergine** function in pigmentation without detectable piRNA production in this context. - Both **Kdm5** and **Piwi** act not only in the pupal abdominal epidermis but also in the **pupal fat body**, indicating a role for fat body signaling or function in female abdominal pigmentation regulation. - The work expands the regulatory network controlling pigmentation genes, assigns a novel somatic role to Kdm5 and Piwi, and highlights a previously underappreciated contribution of the pupal fat body to female abdominal pigmentation. Specific experimental details and quantitative results were not reported in the source abstract.
## Clinical Analysis & Structured Key Points
[Skip to main content](https://www.biorxiv.org/content/10.64898/2026.08.27.747613v1?rss=1#main-content) [![bioRxiv](https://www.biorxiv.org/sites/default/files/biorxiv_article_logo.jpg)](https://www.biorxiv.org/) * [Home](https://www.biorxiv.org/) * [Submit](https://www.biorxiv.org/submit-a-manuscript) * [FAQ](https://www.biorxiv.org/about/FAQ) * [Blog](https://connect.biorxiv.org/news/) * [ALERTS / RSS](https://www.biorxiv.org/content/alertsrss) * [Resources](https://connect.biorxiv.org/resources/) * [About](https://www.biorxiv.org/content/about-biorxiv) * [Channels](https://www.biorxiv.org/content/10.64898/2026.08.27.747613v1?rss=1) * [4D Nucleome](https://connect.biorxiv.org/relate/content/66) * [Academia Sinica](https://connect.biorxiv.org/group/content/38) * [Advances in Genome Biology and Technology (AGBT) General Meeting 2016 #AGBT16](https://connect.biorxiv.org/relate/content/10) * [Albert Einstein College of Medicine](https://connect.biorxiv.org/group/content/4) * [Aligning Science Across Parkinson's (ASAP)](https://connect.biorxiv.org/relate/content/195) * [Allen Institute for Cell Science](https://connect.biorxiv.org/relate/content/71) * [Arc Institute](https://connect.biorxiv.org/relate/content/224) * [Babraham Institute](https://connect.biorxiv.org/relate/content/197) * [BICCN/BICAN](https://connect.biorxiv.org/relate/content/208) * [BioImaging North America](https://connect.biorxiv.org/relate/content/194) * [Biology of Genomes 2016 #BOG16](https://connect.biorxiv.org/relate/content/19) * [Breakthrough Discoveries for Thriving with Bipolar Disorder](https://connect.biorxiv.org/relate/content/236) * [Brown University](https://connect.biorxiv.org/group/content/9) * [California Institute of Technology](https://connect.biorxiv.org/group/content/32) * [Carnegie Mellon University](https://connect.biorxiv.org/group/content/19) * [Case Western Reserve University](https://connect.biorxiv.org/group/content/36) * [Central Oxford Structural Microscopy and Imaging](https://connect.biorxiv.org/relate/content/143) * [Centre for Microbiology and Environmental Systems Science](https://connect.biorxiv.org/relate/content/190) * [Chan Zuckerberg Biohub](https://connect.biorxiv.org/relate/content/121) * [Columbia University](https://connect.biorxiv.org/group/content/27) * [Donders Institute for Brain, Cognition and Behaviour](https://connect.biorxiv.org/relate/content/184) * [DREAM](https://connect.biorxiv.org/relate/content/153) * [Drug Development and Clinical Therapeutics](https://connect.biorxiv.org/relate/content/52) * [ENCODE](https://connect.biorxiv.org/relate/content/177) * [Ernst Strüngmann Institute (ESI) for Neuroscience](https://connect.biorxiv.org/relate/content/211) * [European Molecular Biology Laboratory (EMBL)](https://connect.biorxiv.org/relate/content/159) * [Francis Crick Institute](https://connect.biorxiv.org/relate/content/88) * [Fred Hutchinson Cancer Center](https://connect.biorxiv.org/group/content/28) * [Georgia Institute of Technology](https://connect.biorxiv.org/group/content/20) * [Harvard Program in Therapeutic Sciences](https://connect.biorxiv.org/relate/content/151) * [The Howard Hughes Medical Institute (HHMI)](https://connect.biorxiv.org/relate/content/227) * [Human Cell Atlas](https://connect.biorxiv.org/relate/content/163) * [Human Pangenome Reference Consortium (HPRC)](https://connect.biorxiv.org/relate/content/215) * [IMO Workshop](https://connect.biorxiv.org/relate/content/8) * [Impact of Genomic Variation on Function (IGVF)](https://connect.biorxiv.org/relate/content/214) * [Institut Pasteur](https://connect.biorxiv.org/relate/content/235/channel) * [Institute of Science and Technology Austria](https://connect.biorxiv.org/group/content/12) * [International Human Epigenome Consortium (IHEC)](https://connect.biorxiv.org/relate/content/219) * [International Mouse Phenotyping Consortium (IMPC)](https://connect.biorxiv.org/relate/content/185) * [Iowa State University](https://connect.biorxiv.org/group/content/6) * [Johns Hopkins University](https://connect.biorxiv.org/group/content/47) * [Mathematical Oncology](https://connect.biorxiv.org/relate/content/1) * [Micron Oxford](https://connect.biorxiv.org/relate/content/116) * [Michigan State University](https://connect.biorxiv.org/group/content/7) * [Morgridge Institute](https://connect.biorxiv.org/group/content/57) * [National Taiwan University](https://connect.biorxiv.org/group/content/39) * [NCI Cancer Systems Biology Consortium](https://connect.biorxiv.org/relate/content/210) * [NF Open Science Initiative](https://connect.biorxiv.org/relate/content/213) * [NCI Human Tumor Atlas Network](https://connect.biorxiv.org/relate/content/171) * [Neuromatch Conference](https://connect.biorxiv.org/relate/content/209) * [NeurotechEU](https://connect.biorxiv.org/relate/content/189) * [North Carolina State University](https://connect.biorxiv.org/group/content/37) * [Northeastern University](https://connect.biorxiv.org/group/content/54) * [Oregon Health & Sciences University](https://connect.biorxiv.org/group/content/22) * [RNA Therapeutics Institute at UMass Chan Med School](https://connect.biorxiv.org/relate/content/243) * [Rosetta Commons](https://connect.biorxiv.org/relate/content/191) * [Rutgers University](https://connect.biorxiv.org/group/content/31) * [SeroNet](https://connect.biorxiv.org/relate/content/192) * [Simons Foundation Autism Research Initiative (SFARI)](https://connect.biorxiv.org/relate/content/74) * [Society for Molecular Biology and Evolution #SMBE2016](https://connect.biorxiv.org/relate/content/29) * [Somatic Cell Genome Editing Program](https://connect.biorxiv.org/relate/content/167) * [Somatic Mosaicism across the Human Tissues Network](https://connect.biorxiv.org/relate/content/222) * [SPARC](https://connect.biorxiv.org/relate/content/187) * [Stowers Institute for Medical Research](https://connect.biorxiv.org/group/content/3) * [Stockholm University](https://connect.biorxiv.org/group/content/14) * [Tel Aviv University](https://connect.biorxiv.org/group/content/8) * [The Michael J. Fox Foundation](https://connect.biorxiv.org/relate/content/207) * [The Rockefeller University](https://connect.biorxiv.org/group/content/2) * [The Sainsbury Laboratory](https://connect.biorxiv.org/relate/content/98) * [The Whitehead Institute](https://connect.biorxiv.org/relate/content/212) * [University of Connecticut Health Center](https://connect.biorxiv.org/group/content/16) * [University of California, Berkeley](https://connect.biorxiv.org/group/content/13) * [University of California, San Diego](https://connect.biorxiv.org/group/content/41) * [University of California, San Francisco](https://connect.biorxiv.org/group/content/10) * [University of Chicago](https://connect.biorxiv.org/group/content/42) * [University of Geneva](https://connect.biorxiv.org/group/content/26) * [University of Guelph](https://connect.biorxiv.org/group/content/23) * [University of Hong Kong](https://connect.biorxiv.org/group/content/29) * [University of Illinois Chicago](https://connect.biorxiv.org/group/content/43) * [University of Iowa](https://connect.biorxiv.org/group/content/24) * [University of Kansas](https://connect.biorxiv.org/group/content/33) * [University of Massachusetts Chan Medical School](https://connect.biorxiv.org/group/content/44) * [University of New South Wales](https://connect.biorxiv.org/group/content/25) * [University of Ottawa](https://connect.biorxiv.org/group/content/35) * [University of Sydney](https://connect.biorxiv.org/group/content/50) * [Vanderbilt University](https://connect.biorxiv.org/group/content/30) * [Vienna BioCenter](https://connect.biorxiv.org/relate/content/186) * [Washington University in St. Louis](https://connect.biorxiv.org/group/content/11) * [Weizmann Institute of Science](https://connect.biorxiv.org/group/content/15) * [Yale University](https://connect.biorxiv.org/group/content/53) Search for this keyword [Advanced Search](https://www.biorxiv.org/search) New Results Follow this preprint # The histone demethylase Kdm5 and the ARGONAUTE proteins Piwi and Aubergine regulate female abdominal pigmentation in Drosophila melanogaster Raphael Narbey, Sandra De castro, [ View ORCID Profile](http://orcid.org/0000-0001-7577-2329)Sophie Louvet-Vallée, Michel Gho, [ View ORCID Profile](http://orcid.org/0000-0003-1354-240X)Frédérique Peronnet, [ View ORCID Profile](http://orcid.org/0000-0002-1579-0266)Jean-Michel Gibert, [ View ORCID Profile](http://orcid.org/0000-0001-9428-5243)Emmanuèle Mouchel-Vielh doi: https://doi.org/10.64898/2026.08.27.747613 This article is a preprint and has not been certified by peer review [[what does this mean?](https://www.biorxiv.org/about/FAQ#unrefereed)]. Raphael Narbey 1 Sorbonne Universite; * [Find this author on Google Scholar](https://www.biorxiv.org/lookup/google-scholar?link_type=googlescholar&gs_type=author&author%5B0%5D=Raphael%2BNarbey%2B "Open in new tab") * [Find this author on PubMed](https://www.biorxiv.org/lookup/external-ref?access_num=Narbey%20R&link_type=AUTHORSEARCH "Open in new tab") * [Search for this author on this site](https://www.biorxiv.org/search/author1%3ARaphael%2BNarbey%2B) Sandra De castro 1 Sorbonne Universite; * [Find this author on Google Scholar](https://www.biorxiv.org/lookup/google-scholar?link_type=googlescholar&gs_type=author&author%5B0%5D=Sandra%2BDe%2Bcastro%2B "Open in new tab") * [Find this author on PubMed](https://www.biorxiv.org/lookup/external-ref?access_num=De%20castro%20S&link_type=AUTHORSEARCH "Open in new tab") * [Search for this author on this site](https://www.biorxiv.org/search/author1%3ASandra%2BDe%2Bcastro%2B) Sophie Louvet-Vallée 1 Sorbonne Universite; * [Find this author on Google Scholar](https://www.biorxiv.org/lookup/google-scholar?link_type=googlescholar&gs_type=author&author%5B0%5D=Sophie%2BLouvet-Vall%C3%A9e%2B "Open in new tab") * [Find this author on PubMed](https://www.biorxiv.org/lookup/external-ref?access_num=Louvet-Vall%C3%A9e%20S&link_type=AUTHORSEARCH "Open in new tab") * [Search for this author on this site](https://www.biorxiv.org/search/author1%3ASophie%2BLouvet-Vall%25C3%25A9e%2B) * [ORCID record for Sophie Louvet-Vallée](http://orcid.org/0000-0001-7577-2329 "Open in new tab") Michel Gho 2 Centre National de la Recherche Scientifique (CNRS) * [Find this author on Google Scholar](https://www.biorxiv.org/lookup/google-scholar?link_type=googlescholar&gs_type=author&author%5B0%5D=Michel%2BGho%2B "Open in new tab") * [Find this author on PubMed](https://www.biorxiv.org/lookup/external-ref?access_num=Gho%20M&link_type=AUTHORSEARCH "Open in new tab") * [Search for this author on this site](https://www.biorxiv.org/search/author1%3AMichel%2BGho%2B) Frédérique Peronnet 1 Sorbonne Universite; * [Find this author on Google Scholar](https://www.biorxiv.org/lookup/google-scholar?link_type=googlescholar&gs_type=author&author%5B0%5D=Fr%C3%A9d%C3%A9rique%2BPeronnet%2B "Open in new tab") * [Find this author on PubMed](https://www.biorxiv.org/lookup/external-ref?access_num=Peronnet%20F&link_type=AUTHORSEARCH "Open in new tab") * [Search for this author on this site](https://www.biorxiv.org/search/author1%3AFr%25C3%25A9d%25C3%25A9rique%2BPeronnet%2B) * [ORCID record for Frédérique Peronnet](http://orcid.org/0000-0003-1354-240X "Open in new tab") Jean-Michel Gibert 1 Sorbonne Universite; * [Find this author on Google Scholar](https://www.biorxiv.org/lookup/google-scholar?link_type=googlescholar&gs_type=author&author%5B0%5D=Jean-Michel%2BGibert%2B "Open in new tab") * [Find this author on PubMed](https://www.biorxiv.org/lookup/external-ref?access_num=Gibert%20J&link_type=AUTHORSEARCH "Open in new tab") * [Search for this author on this site](https://www.biorxiv.org/search/author1%3AJean-Michel%2BGibert%2B) * [ORCID record for Jean-Michel Gibert](http://orcid.org/0000-0002-1579-0266 "Open in new tab") Emmanuèle Mouchel-Vielh 1 Sorbonne Universite; * [Find this author on Google Scholar](https://www.biorxiv.org/lookup/google-scholar?link_type=googlescholar&gs_type=author&author%5B0%5D=Emmanu%C3%A8le%2BMouchel-Vielh%2B "Open in new tab") * [Find this author on PubMed](https://www.biorxiv.org/lookup/external-ref?access_num=Mouchel-Vielh%20E&link_type=AUTHORSEARCH "Open in new tab") * [Search for this author on this site](https://www.biorxiv.org/search/author1%3AEmmanu%25C3%25A8le%2BMouchel-Vielh%2B) * [ORCID record for Emmanuèle Mouchel-Vielh](http://orcid.org/0000-0001-9428-5243 "Open in new tab") * For correspondence: emmanuele.mouchel@sorbonne-universite.fr * [Abstract](https://www.biorxiv.org/content/10.64898/2026.08.27.747613v1)[](https://www.biorxiv.org/panels_ajax_tab/biorxiv_tab_art/node:5751116/1) * [Info/History](https://www.biorxiv.org/content/10.64898/2026.08.27.747613v1.article-info)[](https://www.biorxiv.org/panels_ajax_tab/biorxiv_tab_info/node:5751116/1) * [Metrics](https://www.biorxiv.org/content/10.64898/2026.08.27.747613v1.article-metrics)[](https://www.biorxiv.org/panels_ajax_tab/article_tab_metrics/node:5751116/1) * [ Preview PDF](https://www.biorxiv.org/content/10.64898/2026.08.27.747613v1.full.pdf+html)[](https://www.biorxiv.org/panels_ajax_tab/biorxiv_tab_pdf/node:5751116/1) ![Loading](https://www.biorxiv.org/sites/all/modules/contrib/panels_ajax_tab/images/loading.gif) ## Abstract Insect pigmentation is an ecologically critical trait influencing many physiological processes. In _Drosophila melanogaster_ , abdominal pigmentation is sexually dimorphic: males have fully pigmented posterior segments, while females exhibit a posterior melanin stripe. Pigmentation relies on the expression of pigmentation genes that encode enzymes involved in pigment synthesis. These genes are tightly regulated during pupal and young adult stages. To expand the gene regulatory network of pigmentation genes, we conducted an RNAi screen using the _yellow-Gal4_ driver, expressed during the pupal stage in abdominal epidermis. One of the candidates from this screen, _Kdm5_ , encodes a histone demethylase erasing the H3K4me3 histone mark catalyzed by the histone methyl-transferase Trithorax (Trx). We show that _Kdm5_ down-regulation reduces abdominal pigmentation, mimicking _trx_ down-regulation. Kdm5 activates melanin production through regulation of the pigmentation gene _tan_. Transcriptomic analyses reveal that _Kdm5_ and _Trx_ share many targets in pupal abdominal epidermis, including piRNA pathway components such as _piwi_ and _aubergine_. These piRNA components, originally associated with transposon silencing in the germline, also function in some somatic tissues such as the nervous system, the fat body or the gut. We demonstrate that _Piwi_ and _Aubergine_ participate in female abdominal pigmentation establishment, without evident piRNA production. We also show that _Kdm5_ and _Piwi_ act not only in pupal abdominal epidermis but also in pupal fat body. This study therefore expands the regulatory network of pigmentation genes. It identifies a new somatic function for Kdm5 and Piwi and reveals a role for pupal fat body in female abdominal pigmentation regulation. Copyright The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission. bioRxiv and medRxiv thank the following for their generous financial support: > The Chan Zuckerberg Initiative, Cold Spring Harbor Laboratory, the Sergey Brin Family Foundation, California Institute of Technology, Centre National de la Recherche Scientifique, Fred Hutchinson Cancer Center, Imperial College London, Massachusetts Institute of Technology, Stanford University, The University of Edinburgh, University of Washington, and Vrije Universiteit Amsterdam. [Donate to openRxiv ](https://www.zeffy.com/en-US/donation-form/donate-to-make-a-difference-10981) [ Back to top](https://www.biorxiv.org/content/10.64898/2026.08.27.747613v1?rss=1#page) [ Previous](https://www.biorxiv.org/content/10.64898/2026.07.21.739769v2 "MetaClaw: an auditable AI agent for end-to-end, multi-directional metagenomic and multi-omics analysis")[Next ](https://www.biorxiv.org/content/10.64898/2026.08.31.747360v1 "Sobetirome, a thyroid hormone receptor beta agonist, is a potential therapeutic agent for pulmonary fibrosis") Posted September 03, 2026. [ Download PDF](https://www.biorxiv.org/content/10.64898/2026.08.27.747613v1.full.pdf) Print/Save Options [Download PDF](https://www.biorxiv.org/content/biorxiv/early/2026/09/03/2026.08.27.747613.full.pdf)Full Text & In-line FiguresXML [More Info](https://www.biorxiv.org/about/FAQ#PrintOptions "More Information on Print/Save Options") [ Email](https://www.biorxiv.org/ "Email this Article") [ Share](https://www.biorxiv.org/) The histone demethylase Kdm5 and the ARGONAUTE proteins Piwi and Aubergine regulate female abdominal pigmentation in Drosophila melanogaster Raphael Narbey, Sandra De castro, Sophie Louvet-Vallée, Michel Gho, Frédérique Peronnet, Jean-Michel Gibert, Emmanuèle Mouchel-Vielh bioRxiv 2026.08.27.747613; doi: https://doi.org/10.64898/2026.08.27.747613 This article is a preprint and has not been certified by peer review [[what does this mean?](https://www.biorxiv.org/about/FAQ#unrefereed)]. Share This Article: Copy [![Twitter logo](https://www.biorxiv.org/sites/all/modules/highwire/highwire/images/twitter.png)](https://www.biorxiv.org/highwire_log/share/twitter?link=http%3A%2F%2Ftwitter.com%2Fshare%3Furl%3Dhttps%253A%2F%2Fwww.biorxiv.org%2Fcontent%2F10.64898%2F2026.08.27.747613v1%26text%3DThe%2520histone%2520demethylase%2520Kdm5%2520and%2520the%2520ARGONAUTE%2520proteins%2520Piwi%2520and%2520Aubergine%2520regulate%2520female%2520abdominal%2520pigmentation%2520in%2520Drosophila%2520melanogaster "Share this on Twitter") [![Facebook logo](https://www.biorxiv.org/sites/all/modules/highwire/highwire/images/fb-blue.png)](https://www.biorxiv.org/highwire_log/share/facebook?link=http%3A%2F%2Fwww.facebook.com%2Fsharer.php%3Fu%3Dhttps%253A%2F%2Fwww.biorxiv.org%2Fcontent%2F10.64898%2F2026.08.27.747613v1%26t%3DThe%2520histone%2520demethylase%2520Kdm5%2520and%2520the%2520ARGONAUTE%2520proteins%2520Piwi%2520and%2520Aubergine%2520regulate%2520female%2520abdominal%2520pigmentation%2520in%2520Drosophila%2520melanogaster "Share on Facebook") [![LinkedIn logo](https://www.biorxiv.org/sites/all/modules/highwire/highwire/images/linkedin-32px.png)](https://www.biorxiv.org/highwire_log/share/linkedin?link=http%3A%2F%2Fwww.linkedin.com%2FshareArticle%3Fmini%3Dtrue%26url%3Dhttps%253A%2F%2Fwww.biorxiv.org%2Fcontent%2F10.64898%2F2026.08.27.747613v1%26title%3DThe%2520histone%2520demethylase%2520Kdm5%2520and%2520the%2520ARGONAUTE%2520proteins%2520Piwi%2520and%2520Aubergine%2520regulate%2520female%2520abdominal%2520pigmentation%2520in%2520Drosophila%2520melanogaster%26summary%3D%26source%3DbioRxiv "Publish this post to LinkedIn") [![Mendeley logo](https://www.biorxiv.org/sites/all/modules/highwire/highwire/images/mendeley.png)](https://www.biorxiv.org/highwire_log/share/mendeley?link=http%3A%2F%2Fwww.mendeley.com%2Fimport%2F%3Furl%3Dhttps%253A%2F%2Fwww.biorxiv.org%2Fcontent%2F10.64898%2F2026.08.27.747613v1%26title%3DThe%2520histone%2520demethylase%2520Kdm5%2520and%2520the%2520ARGONAUTE%2520proteins%2520Piwi%2520and%2520Aubergine%2520regulate%2520female%2520abdominal%2520pigmentation%2520in%2520Drosophila%2520melanogaster "Share on Mendeley") [ Citation Tools](https://www.biorxiv.org/ "Citation Tools") [ Get QR code](https://con
## Related Clinical Research

- [Open-source 3D-printed vacuum manifold for automated spin-column DNA isolation on Opentrons](https://medichelpline.com/clinical-feed/biorxiv-1-an-open-source-3d-printed-vacuum-manifold-for-automated-dna-isolation-in-the.md)
- [DBL-1/ BMP Smads interact with BLMP-1/PRDM1 to control multiple organismal traits in C. elegans](https://medichelpline.com/clinical-feed/biorxiv-5-dbl-1-bmp-signaling-pathway-smads-and-master-transcription-regulator-blmp-1.md)
- [Eye movement analysis identifies early cognitive impairment in cirrhosis missed by PHES](https://medichelpline.com/clinical-feed/plos-one-12-eye-movement-analysis-detects-early-neurological-alterations-in-cirrhotic.md)
- [8-hour time-restricted eating yields greater weight loss but not consistent blood pressure benefits](https://medichelpline.com/clinical-feed/medical-news-today-0-16-8-intermittent-fasting-best-for-weight-loss-but-not-for-blood-pressure.md)
- [Comparing Melatonin and Magnesium: Which is Better for Sleep?](https://medichelpline.com/clinical-feed/medical-news-today-0-should-you-take-melatonin-or-magnesium-for-sleep-experts-weigh-in.md)

## Navigation
- [← Back to General Feed](https://medichelpline.com/clinical-feed/general.md)
- [← All Clinical Specialties](https://medichelpline.com/clinical-feed.md)
## Medical & Regulatory Disclaimer

> [!CAUTION]
> MedicHelpline content is structured for research, educational, and professional discovery purposes. It does not constitute individual medical advice, clinical diagnosis, or treatment recommendations.
> Always verify dosing, contraindications, and regulatory alerts against official product labeling and primary regulatory sources before clinical decision-making.