---
title: "Spatio-temporal imaging and acute manipulation of endogenous Yap1 in medaka using a Yap1-mGreenLan"
id: "biorxiv-17-spatio-temporal-visualisation-and-manipulation-of-endogenous-yap1-in-medaka"
canonical_url: "https://medichelpline.com/clinical-feed/biorxiv-17-spatio-temporal-visualisation-and-manipulation-of-endogenous-yap1-in-medaka"
content_type: "clinical_feed_article"
specialty: "General"
source_name: "bioRxiv (Biomedical Preprints)"
source_url: "https://www.biorxiv.org/content/10.64898/2026.09.15.751690v1?rss=1"
published_at: "2026-09-21T12:00:00.000Z"
evidence_level: "Verified Feed"
license: "CC-BY-NC-4.0 / Informational Use"
---
# Spatio-temporal imaging and acute manipulation of endogenous Yap1 in medaka using a Yap1-mGreenLan
## Provenance & Clinical Metadata
- **Canonical URL:** https://medichelpline.com/clinical-feed/biorxiv-17-spatio-temporal-visualisation-and-manipulation-of-endogenous-yap1-in-medaka
- **Specialty:** [General](https://medichelpline.com/clinical-feed/general.md)
- **Primary Source:** bioRxiv (Biomedical Preprints)
- **Source URL:** [Original Journal Publication](https://www.biorxiv.org/content/10.64898/2026.09.15.751690v1?rss=1)
- **Published At:** 2026-09-21T12:00:00.000Z
- **Evidence Rating:** Verified Feed
## Executive GIST (TL;DR)
- The study creates an endogenous **Yap1** reporter in medaka by CRISPR/Cas9 knock-in of a **mGreenLantern** tag (Yap1-mGL), enabling in vivo visualisation and manipulation of the protein. - The reporter supports 4D (space and time) analysis of **Yap1** dynamics, including real-time visualisation of expression onset and subcellular localisation. - Reporter fluorescence is detectable before gastrulation, allowing early developmental tracking of **Yap1**. - During somitogenesis the authors observe tissue- and cell type–specific patterns of nuclear versus cytoplasmic **Yap1-mGL** localisation. - In larval stages, spinal cord injury induces upregulation of **Yap1-mGL**, demonstrating the reporter’s utility for regeneration studies. - The team couples endogenous tagging with a **nanobody-based degron** system to acutely degrade Yap1 in vivo, reproducing the known genetic loss-of-function phenotype. - Combining endogenous fluorescent tags with nanobody-driven degradation provides a platform to both visualise and rapidly perturb an endogenous signalling effector, here applied to the conserved **Hippo pathway** effector Yap1. - The work is presented as a preprint and has not undergone peer review; methodological and dataset specifics beyond the abstract were not reported in the source provided.
## Clinical Analysis & Structured Key Points
Spatio-temporal visualisation and manipulation of endogenous Yap1 in medaka | bioRxiv Skip to main content New Results Spatio-temporal visualisation and manipulation of endogenous Yap1 in medaka Ali Seleit , Yoichi Asaoka , Simon Knoblich , Luc Lederer , Chiharu Oozono , Atsunori Oga , Akihiro Oiso , Makoto Furutani-Seiki , View ORCID Profile Alexander Aulehla doi: https://doi.org/10.64898/2026.09.15.751690 Ali Seleit 1 CIBSS; Find this author on Google Scholar Find this author on PubMed Search for this author on this site Yoichi Asaoka 2 Yamaguchi University; Find this author on Google Scholar Find this author on PubMed Search for this author on this site Simon Knoblich 3 EMBL Heidelberg; Find this author on Google Scholar Find this author on PubMed Search for this author on this site Luc Lederer 4 EMBL; Find this author on Google Scholar Find this author on PubMed Search for this author on this site Chiharu Oozono 5 Graduate School of Medicine, Yamaguchi University; Find this author on Google Scholar Find this author on PubMed Search for this author on this site Atsunori Oga 5 Graduate School of Medicine, Yamaguchi University; Find this author on Google Scholar Find this author on PubMed Search for this author on this site Akihiro Oiso 5 Graduate School of Medicine, Yamaguchi University; Find this author on Google Scholar Find this author on PubMed Search for this author on this site Makoto Furutani-Seiki 6 Graduate School of Medicine, Yamaguchi University, 755-8505 , Japan Find this author on Google Scholar Find this author on PubMed Search for this author on this site Alexander Aulehla 4 EMBL; Find this author on Google Scholar Find this author on PubMed Search for this author on this site ORCID record for Alexander Aulehla For correspondence: aulehla{at}embl.de Abstract Info/History Metrics Preview PDF Abstract A key requirement to address fundamental questions in developmental biology is the ability to visualise and manipulate endogenous signalling proteins in vivo. Here we generate such an enabling experimental tool for the highly conserved Hippo pathway and its transcriptional effector Yap1, which converts mechanical and chemical cues into transcriptional output during embryogenesis and regeneration. We generate and characterise a Yap1- mGreenLantern (Yap1-mGL) endogenous CRISPR/Cas9-mediated knock-in line that enables 4D analysis of Yap1 dynamics in medaka. We resolve Yap1- mGL expression and nuclear/cytoplasmic localisation in real time, revealing the onset of reporter fluorescence before gastrulation, tissue and cell-type specific Yap1-mGL localisation during somitogenesis and injury-induced Yap1 upregulation during larval spinal cord regeneration. Using the nanobody-based degron system, we degrade the Yap1 protein in vivo, recapitulating the genetic loss-of-function mutant phenotype. Combining endogenous tags with nanobody-based degrons offers a powerful approach to visualise and acutely perturb endogenous protein function in vivo. Copyright The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license . Back to top Previous Posted September 21, 2026. Download PDF Email Thank you for your interest in spreading the word about bioRxiv. NOTE: Your email address is requested solely to identify you as the sender of this article. Your Email * Your Name * Send To * Enter multiple addresses on separate lines or separate them with commas. You are going to email the following Spatio-temporal visualisation and manipulation of endogenous Yap1 in medaka Message Subject (Your Name) has forwarded a page to you from bioRxiv Message Body (Your Name) thought you would like to see this page from the bioRxiv website. Your Personal Message CAPTCHA This question is for testing whether or not you are a human visitor and to prevent automated spam submissions. Share Spatio-temporal visualisation and manipulation of endogenous Yap1 in medaka Ali Seleit , Yoichi Asaoka , Simon Knoblich , Luc Lederer , Chiharu Oozono , Atsunori Oga , Akihiro Oiso , Makoto Furutani-Seiki , Alexander Aulehla bioRxiv 2026.09.15.751690; doi: https://doi.org/10.64898/2026.09.15.751690 Share This Article: Copy Citation Tools Spatio-temporal visualisation and manipulation of endogenous Yap1 in medaka Ali Seleit , Yoichi Asaoka , Simon Knoblich , Luc Lederer , Chiharu Oozono , Atsunori Oga , Akihiro Oiso , Makoto Furutani-Seiki , Alexander Aulehla bioRxiv 2026.09.15.751690; doi: https://doi.org/10.64898/2026.09.15.751690 Citation Manager Formats BibTeX Bookends EasyBib EndNote (tagged) EndNote 8 (xml) Medlars Mendeley Papers RefWorks Tagged Ref Manager RIS Zotero Tweet Widget Facebook Like Google Plus One Subject Areas All Articles Animal Behavior and Cognition (8014) Biochemistry (18743) Bioengineering (14890) Bioinformatics (44443) Biophysics (22609) Cancer Biology (19728) Cell Biology (26906) Clinical Trials (138) Developmental Biology (13969) Ecology (21008) Epidemiology (2067) Evolutionary Biology (25457) Genetics (16169) Genomics (23512) Immunology (18714) Microbiology (42520) Molecular Biology (18063) Neuroscience (93474) Paleontology (700) Pathology (2981) Pharmacology and Toxicology (5098) Physiology (8115) Plant Biology (16000) Scientific Communication and Education (2095) Synthetic Biology (4560) Systems Biology (10236) Zoology (2391)
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