---
title: "Amoxicillin Differentially Alters Extracellular Vesicle Production by Bifidobacterium longum and L"
id: "biorxiv-6-dissociated-responses-of-vesiculogenesis-and-amoxicillin-impact-on"
canonical_url: "https://medichelpline.com/clinical-feed/biorxiv-6-dissociated-responses-of-vesiculogenesis-and-amoxicillin-impact-on"
content_type: "clinical_feed_article"
specialty: "Infectious Disease"
source_name: "bioRxiv (Biomedical Preprints)"
source_url: "https://www.biorxiv.org/content/10.64898/2026.07.27.740896v1?rss=1"
published_at: "2026-07-27T12:00:00.000Z"
evidence_level: "Verified Feed"
license: "CC-BY-NC-4.0 / Informational Use"
---
# Amoxicillin Differentially Alters Extracellular Vesicle Production by Bifidobacterium longum and L
## Provenance & Clinical Metadata
- **Canonical URL:** https://medichelpline.com/clinical-feed/biorxiv-6-dissociated-responses-of-vesiculogenesis-and-amoxicillin-impact-on
- **Specialty:** [Infectious Disease](https://medichelpline.com/clinical-feed/infectious-disease.md)
- **Primary Source:** bioRxiv (Biomedical Preprints)
- **Source URL:** [Original Journal Publication](https://www.biorxiv.org/content/10.64898/2026.07.27.740896v1?rss=1)
- **Published At:** 2026-07-27T12:00:00.000Z
- **Evidence Rating:** Verified Feed
## Executive GIST (TL;DR)
- The study examined how **amoxicillin** affects production and protein content of **extracellular vesicles (EVs)** from two early gut colonizers: **Bifidobacterium longum** and **Lactiplantibacillus plantarum**. - Authors developed and used a robust, reproducible in-house workflow to extract and purify **bacterial EVs (bEVs)** from both Gram-positive strains. - The neonatal period is emphasized as a critical window when primo-colonizing bacteria contribute to maturation of digestive, immune, and neural systems; perturbations such as antibiotics may alter these interactions. - Findings show a dissociated response between species: **B. longum** increased EV production when exposed to amoxicillin, while **L. plantarum** did not show the same enhancement. - Proteovesiculome analysis revealed that amoxicillin-treated **B. longum** EVs were enriched and more diverse in protein content compared with non-treated controls. - Conversely, **L. plantarum** EVs from amoxicillin-treated cells showed a shift toward loss of protein richness. - The results suggest species-specific regulation of vesiculogenesis and cargo loading in response to antibiotic exposure, with potential implications for microbiota-host communication after neonatal antibiotic therapies. - The article is a preprint and has not been peer reviewed; methodological and contextual details beyond the abstract are reported in the source but full peer-reviewed confirmation is pending.
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Anaïs Halbert 1 INRAE; * [Find this author on Google Scholar](https://www.biorxiv.org/lookup/google-scholar?link_type=googlescholar&gs_type=author&author%5B0%5D=Ana%C3%AFs%2BHalbert%2B "Open in new tab") * [Find this author on PubMed](https://www.biorxiv.org/lookup/external-ref?access_num=Halbert%20A&link_type=AUTHORSEARCH "Open in new tab") * [Search for this author on this site](https://www.biorxiv.org/search/author1%3AAna%25C3%25AFs%2BHalbert%2B) Amandine Dupuy 2 INSERM; * [Find this author on Google Scholar](https://www.biorxiv.org/lookup/google-scholar?link_type=googlescholar&gs_type=author&author%5B0%5D=Amandine%2BDupuy%2B "Open in new tab") * [Find this author on PubMed](https://www.biorxiv.org/lookup/external-ref?access_num=Dupuy%20A&link_type=AUTHORSEARCH "Open in new tab") * [Search for this author on this site](https://www.biorxiv.org/search/author1%3AAmandine%2BDupuy%2B) Lisa Wallart 3 CNRS; * [Find this author on Google Scholar](https://www.biorxiv.org/lookup/google-scholar?link_type=googlescholar&gs_type=author&author%5B0%5D=Lisa%2BWallart%2B "Open in new tab") * [Find this author on PubMed](https://www.biorxiv.org/lookup/external-ref?access_num=Wallart%20L&link_type=AUTHORSEARCH "Open in new tab") * [Search for this author on this site](https://www.biorxiv.org/search/author1%3ALisa%2BWallart%2B) Sophie Brouard 4 Nantes University; * [Find this author on Google Scholar](https://www.biorxiv.org/lookup/google-scholar?link_type=googlescholar&gs_type=author&author%5B0%5D=Sophie%2BBrouard%2B "Open in new tab") * [Find this author on PubMed](https://www.biorxiv.org/lookup/external-ref?access_num=Brouard%20S&link_type=AUTHORSEARCH "Open in new tab") * [Search for this author on this site](https://www.biorxiv.org/search/author1%3ASophie%2BBrouard%2B) * [ORCID record for Sophie Brouard](http://orcid.org/0000-0002-6398-1315 "Open in new tab") Julie Hardouin 5 University of Rouen * [Find this author on Google Scholar](https://www.biorxiv.org/lookup/google-scholar?link_type=googlescholar&gs_type=author&author%5B0%5D=Julie%2BHardouin%2B "Open in new tab") * [Find this author on PubMed](https://www.biorxiv.org/lookup/external-ref?access_num=Hardouin%20J&link_type=AUTHORSEARCH "Open in new tab") * [Search for this author on this site](https://www.biorxiv.org/search/author1%3AJulie%2BHardouin%2B) * [ORCID record for Julie Hardouin](http://orcid.org/0000-0003-4588-9811 "Open in new tab") Hervé Modeste Blottière 1 INRAE; * [Find this author on Google Scholar](https://www.biorxiv.org/lookup/google-scholar?link_type=googlescholar&gs_type=author&author%5B0%5D=Herv%C3%A9%2BModeste%2BBlotti%C3%A8re%2B "Open in new tab") * [Find this author on PubMed](https://www.biorxiv.org/lookup/external-ref?access_num=Blotti%C3%A8re%20HM&link_type=AUTHORSEARCH "Open in new tab") * [Search for this author on this site](https://www.biorxiv.org/search/author1%3AHerv%25C3%25A9%2BModeste%2BBlotti%25C3%25A8re%2B) * [ORCID record for Hervé Modeste Blottière](http://orcid.org/0000-0002-8390-0607 "Open in new tab") Odile Tresse 1 INRAE; * [Find this author on Google Scholar](https://www.biorxiv.org/lookup/google-scholar?link_type=googlescholar&gs_type=author&author%5B0%5D=Odile%2BTresse%2B "Open in new tab") * [Find this author on PubMed](https://www.biorxiv.org/lookup/external-ref?access_num=Tresse%20O&link_type=AUTHORSEARCH "Open in new tab") * [Search for this author on this site](https://www.biorxiv.org/search/author1%3AOdile%2BTresse%2B) * [ORCID record for Odile Tresse](http://orcid.org/0000-0001-9833-2510 "Open in new tab") * For correspondence: odile.tresse@inrae.fr * [Abstract](https://www.biorxiv.org/content/10.64898/2026.07.27.740896v1)[](https://www.biorxiv.org/panels_ajax_tab/biorxiv_tab_art/node:5658654/1) * [Info/History](https://www.biorxiv.org/content/10.64898/2026.07.27.740896v1.article-info)[](https://www.biorxiv.org/panels_ajax_tab/biorxiv_tab_info/node:5658654/1) * [Metrics](https://www.biorxiv.org/content/10.64898/2026.07.27.740896v1.article-metrics)[](https://www.biorxiv.org/panels_ajax_tab/article_tab_metrics/node:5658654/1) * [Data/Code](https://www.biorxiv.org/content/10.64898/2026.07.27.740896v1.external-links)[](https://www.biorxiv.org/panels_ajax_tab/article_tab_data_code/node:5658654/1) * [ Preview PDF](https://www.biorxiv.org/content/10.64898/2026.07.27.740896v1.full.pdf+html)[](https://www.biorxiv.org/panels_ajax_tab/biorxiv_tab_pdf/node:5658654/1) ![Loading](https://www.biorxiv.org/sites/all/modules/contrib/panels_ajax_tab/images/loading.gif) ## Abstract Bacterial extracellular vesicles (bEVs) have emerged as important mediators of microbiota-host interplay through the transport of active biomolecules, namely cargos, far from their release location. The neonatal period represents a critical window for the establishment of the gut microbiota and subsequent sustainable symbiotic communication. The gut primo-colonizing bacteria, including bifidobacteria and lactobacilli, likely contribute to the impact on the digestive, immune and neuron system maturation. However, exposures and experiences during this early stage may influence the development of health and diseases later on in life by altering these primo-interactions. As antibiotherapies are frequent in the postnatal period and associated to microbiota disorders, we evaluated the impact of amoxicillin on first colonizing Gram positive-derived EVs, using a robust and reproductible in-house workflow for the extraction and purification bEVs from _Bifidobacterium longum_ and _Lactiplantibacillus plantarum_. The EVs production and the proteovesiculome profiles under amoxicillin treatment were compared. The results pointed out a dissociated response in the EVs release process and their regulation by amoxicillin according to strain with an enhance production of EVs for _B. longum_ under amoxicillin. In addition, the proteovesicular analyses indicate that the vesicular protein profile was enriched and more diverse in _B. longum_ -derived EVs from amoxicillin-treated cells than those from non-treated cells while the content shift in _L. plantarum_ -derived EVs in amoxicillin-treated cells was in favor of protein richness loss. Overall, this study opens new avenues considering the impact of antibiotic therapies in the neonatal period on EVs derived from benefit Gram-positive gut bacteria. ### Competing Interest Statement The authors have declared no competing interest. ## Footnotes * ## Funder Information Declared Santé-DIGE, 34001344 Copyright The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a [CC-BY-NC-ND 4.0 International license](http://creativecommons.org/licenses/by-nc-nd/4.0/). bioRxiv and medRxiv thank the following for their generous financial support: > The Chan Zuckerberg Initiative, Cold Spring Harbor Laboratory, the Sergey Brin Family Foundation, California Institute of Technology, Centre National de la Recherche Scientifique, Fred Hutchinson Cancer Center, Imperial College London, Massachusetts Institute of Technology, Stanford University, The University of Edinburgh, University of Washington, and Vrije Universiteit Amsterdam. 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[ Download PDF](https://www.biorxiv.org/content/10.64898/2026.07.27.740896v1.full.pdf) Print/Save Options [Download PDF](https://www.biorxiv.org/content/biorxiv/early/2026/07/27/2026.07.27.740896.full.pdf)Full Text & In-line FiguresXML [More Info](https://www.biorxiv.org/about/FAQ#PrintOptions "More Information on Print/Save Options") [ Data/Code](https://www.biorxiv.org/content/early/2026/07/27/2026.07.27.740896.external-links) [ Email](https://www.biorxiv.org/ "Email this Article") [ Share](https://www.biorxiv.org/) Dissociated responses of vesiculogenesis and amoxicillin impact on extracellular vesicle production of first gut bacterial colonizers _Bifidobacterium longum_ and _Lactiplantibacillus plantarum_ Anaïs Halbert, Amandine Dupuy, Lisa Wallart, Sophie Brouard, Julie Hardouin, Hervé Modeste Blottière, Odile Tresse bioRxiv 2026.07.27.740896; doi: https://doi.org/10.64898/2026.07.27.740896 This article is a preprint and has not been certified by peer review [[what does this mean?](https://www.biorxiv.org/about/FAQ#unrefereed)]. 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