---
title: "Bacterial lipid effectors impair autophagy by causing lysosomal dysfunction"
id: "biorxiv-20-bacterial-lipid-effectors-subvert-host-autophagic-flux-by-inducing-lysosomal"
canonical_url: "https://medichelpline.com/clinical-feed/biorxiv-20-bacterial-lipid-effectors-subvert-host-autophagic-flux-by-inducing-lysosomal"
content_type: "clinical_feed_article"
specialty: "Infectious Disease"
source_name: "bioRxiv (Biomedical Preprints)"
source_url: "https://www.biorxiv.org/content/10.64898/2026.09.18.752599v1?rss=1"
published_at: "2026-09-22T12:00:00.000Z"
evidence_level: "Verified Feed"
license: "CC-BY-NC-4.0 / Informational Use"
---
# Bacterial lipid effectors impair autophagy by causing lysosomal dysfunction
## Provenance & Clinical Metadata
- **Canonical URL:** https://medichelpline.com/clinical-feed/biorxiv-20-bacterial-lipid-effectors-subvert-host-autophagic-flux-by-inducing-lysosomal
- **Specialty:** [Infectious Disease](https://medichelpline.com/clinical-feed/infectious-disease.md)
- **Primary Source:** bioRxiv (Biomedical Preprints)
- **Source URL:** [Original Journal Publication](https://www.biorxiv.org/content/10.64898/2026.09.18.752599v1?rss=1)
- **Published At:** 2026-09-22T12:00:00.000Z
- **Evidence Rating:** Verified Feed
## Executive GIST (TL;DR)
- Authors identify HlyF and CprA as a family of cytoplasmic bacterial enzymes that drive virulence by generating outer membrane vesicles (OMVs) enriched in toxic bioactive **lipids**. - OMVs produced by bacteria expressing HlyF/CprA impair host **autophagic flux** by targeting lysosomal function rather than upstream autophagosome formation. - OMV-associated lipids disrupt autophagosome–lysosome fusion and induce hallmarks of lysosomal dysfunction: defective acidification, **cholesterol** accumulation, and lysosomal membrane permeabilization. - These lipid-mediated alterations compromise lysosomal integrity and block autophagic degradation, subverting a central host clearance pathway. - The study highlights a previously unrecognized mechanism of virulence mediated by bacterial lipid effectors and notes the widespread expression of HlyF/CprA homologs across diverse pathogens. - Findings are reported in a preprint and have not been peer reviewed; authors declared no competing interests and funding sources were listed from French national agencies.
## Clinical Analysis & Structured Key Points
Bacterial lipid effectors subvert host autophagic flux by inducing lysosomal dysfunction | bioRxiv Skip to main content New Results Bacterial lipid effectors subvert host autophagic flux by inducing lysosomal dysfunction Camille PIN , Christelle MARRAULD , Priscilla BRANCHU , Chrystelle BONNART , Corinne ROLLAND , Lhorane LOBJOIS , Simon LACHAMBRE , Frederic TAIEB , View ORCID Profile Eric Oswald , View ORCID Profile Laure DAVID doi: https://doi.org/10.64898/2026.09.18.752599 Camille PIN INSERM Find this author on Google Scholar Find this author on PubMed Search for this author on this site Christelle MARRAULD INSERM Find this author on Google Scholar Find this author on PubMed Search for this author on this site Priscilla BRANCHU INSERM Find this author on Google Scholar Find this author on PubMed Search for this author on this site Chrystelle BONNART INSERM Find this author on Google Scholar Find this author on PubMed Search for this author on this site Corinne ROLLAND INSERM Find this author on Google Scholar Find this author on PubMed Search for this author on this site Lhorane LOBJOIS INSERM Find this author on Google Scholar Find this author on PubMed Search for this author on this site Simon LACHAMBRE INSERM Find this author on Google Scholar Find this author on PubMed Search for this author on this site Frederic TAIEB INSERM Find this author on Google Scholar Find this author on PubMed Search for this author on this site Eric Oswald INSERM Find this author on Google Scholar Find this author on PubMed Search for this author on this site ORCID record for Eric Oswald For correspondence: eric.oswald{at}inserm.fr Laure DAVID INSERM Find this author on Google Scholar Find this author on PubMed Search for this author on this site ORCID record for Laure DAVID Abstract Info/History Metrics Preview PDF Abstract HlyF and CprA, virulence factors from Escherichia coli and Pseudomonas aeruginosa respectively, constitute a previously unrecognized family of cytoplasmic enzymes that drive virulence through the production of outer membrane vesicles (OMVs) enriched in toxic bioactive lipids. Here, we show that OMVs of bacteria producing HlyF/CprA impair host autophagic flux by targeting lysosomal function. Specifically, these OMV-associated lipids disrupt autophagosome-lysosome fusion and induce hallmark features of lysosomal dysfunctions, including defective acidification, cholesterol accumulation, and membrane permeabilization. These lipid-mediated alterations compromise lysosomal integrity and block autophagic degradation. Our findings reveal a novel mechanism by which bacterial lipids subvert lysosomal functions and host clearance pathways. Given the widespread expression of HlyF/CprA homologs across diverse pathogens, this work highlights lipid effectors as key determinants of pathogenesis. Competing Interest Statement The authors have declared no competing interest. Funder Information Declared French National Research Agency projects , SMERSEC (ANR-20-CE18-0016) , VeSPath (ANR-23-CE14-0070) doctoral fellowship provided by the French Ministry of Higher Education and Research Copyright The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license . Back to top Previous Next Posted September 22, 2026. Download PDF Email Thank you for your interest in spreading the word about bioRxiv. NOTE: Your email address is requested solely to identify you as the sender of this article. Your Email * Your Name * Send To * Enter multiple addresses on separate lines or separate them with commas. 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Share Bacterial lipid effectors subvert host autophagic flux by inducing lysosomal dysfunction Camille PIN , Christelle MARRAULD , Priscilla BRANCHU , Chrystelle BONNART , Corinne ROLLAND , Lhorane LOBJOIS , Simon LACHAMBRE , Frederic TAIEB , Eric Oswald , Laure DAVID bioRxiv 2026.09.18.752599; doi: https://doi.org/10.64898/2026.09.18.752599 Share This Article: Copy Citation Tools Bacterial lipid effectors subvert host autophagic flux by inducing lysosomal dysfunction Camille PIN , Christelle MARRAULD , Priscilla BRANCHU , Chrystelle BONNART , Corinne ROLLAND , Lhorane LOBJOIS , Simon LACHAMBRE , Frederic TAIEB , Eric Oswald , Laure DAVID bioRxiv 2026.09.18.752599; doi: https://doi.org/10.64898/2026.09.18.752599 Citation Manager Formats BibTeX Bookends EasyBib EndNote (tagged) EndNote 8 (xml) Medlars Mendeley Papers RefWorks Tagged Ref Manager RIS Zotero Tweet Widget Facebook Like Google Plus One Subject Areas All Articles Animal Behavior and Cognition (8019) Biochemistry (18749) Bioengineering (14905) Bioinformatics (44471) Biophysics (22615) Cancer Biology (19733) Cell Biology (26913) Clinical Trials (138) Developmental Biology (13974) Ecology (21013) Epidemiology (2067) Evolutionary Biology (25466) Genetics (16177) Genomics (23527) Immunology (18720) Microbiology (42540) Molecular Biology (18075) Neuroscience (93552) Paleontology (701) Pathology (2984) Pharmacology and Toxicology (5102) Physiology (8123) Plant Biology (16006) Scientific Communication and Education (2095) Synthetic Biology (4562) Systems Biology (10239) Zoology (2392)
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