---
title: "Herbal cake-separated moxibustion plus atorvastatin reduces atherosclerosis in ApoE-/- mice via in"
id: "pubmed-42621708"
canonical_url: "https://medichelpline.com/clinical-feed/pubmed-42621708"
content_type: "clinical_feed_article"
specialty: "Pharmacology"
source_name: "PubMed / NCBI"
source_url: "https://pubmed.ncbi.nlm.nih.gov/42621708/"
doi: "10.13702/j.1000-0607.20250861"
published_at: "2026-08-25T00:00:00.000Z"
evidence_level: "English Abstract"
license: "CC-BY-NC-4.0 / Informational Use"
---
# Herbal cake-separated moxibustion plus atorvastatin reduces atherosclerosis in ApoE-/- mice via in
## Provenance & Clinical Metadata
- **Canonical URL:** https://medichelpline.com/clinical-feed/pubmed-42621708
- **Specialty:** [Pharmacology](https://medichelpline.com/clinical-feed/pharmacology.md)
- **Primary Source:** PubMed / NCBI
- **Source URL:** [Original Journal Publication](https://pubmed.ncbi.nlm.nih.gov/42621708/)
- **DOI:** [10.13702/j.1000-0607.20250861](https://doi.org/10.13702%2Fj.1000-0607.20250861)
- **Published At:** 2026-08-25T00:00:00.000Z
- **Evidence Rating:** English Abstract
## Executive GIST (TL;DR)
- Objective: The study evaluated whether **herbal cake-separated moxibustion** combined with **atorvastatin** affects lipid metabolism, aortic pathology, plaque deposition, and the **integrin/YAP/JNK** signaling pathway in ApoE-/- atherosclerotic mice, and explored possible preventive and therapeutic mechanisms. - Design: Male C57BL/6J mice served as blank controls; male ApoE-/- mice were randomized into model, medicine (atorvastatin), herbal cake-separated moxibustion, and combined treatment groups (n = 9 per group). A high-fat diet induced atherosclerosis; interventions lasted 8 weeks. - Interventions: Moxibustion was applied at acupoints Danzhong (CV17) and Shenque (CV8) every other day, 3 times weekly. Atorvastatin calcium was given by gavage at 3 mg·kg-1·d-1 once daily. The combined group received both therapies concurrently. - Assessments: Body weight before and after treatment; aortic histology by HE staining; aortic lipid area by Oil Red O; serum TG, TC, LDL-C, HDL-C by biochemical analysis; serum VEGF, ET-1, IL-6, IL-8, TNF-α by ELISA; serum NO by colorimetric assay; aortic protein expression (integrin αVβ3, YAP, p-YAP, p-JNK1/2, ICAM-1, VCAM-1) by Western blot. - Key model findings: Compared with blank controls, ApoE-/- model mice showed increased body weight, thickened aortic intima with foam cells and lipid deposition, smooth muscle cell hypertrophy/edema, elastic fiber thinning and membrane rupture, greater arterial plaque area, higher serum TC, TG, LDL-C, IL-6, IL-8, TNF-α, ET-1, VEGF, lower HDL-C and NO, increased aortic integrin αVβ3, YAP, p-JNK1/2, ICAM-1, VCAM-1 and decreased p-YAP (all P < 0.01). - Treatment effects: Medicine, moxibustion, and combined groups all reduced body weight and improved aortic morphology and most biochemical and inflammatory markers versus model (P < 0.05 or P < 0.01). Atorvastatin and combined therapy produced greater plaque reduction, increased NO, lowered LDL-C, ET-1, VEGF, and reduced aortic p-JNK1/2, ICAM-1, VCAM-1 compared with moxibustion alone. - Atorvastatin-specific effects: Atorvastatin decreased TC, TG, IL-6, IL-8, TNF-α, increased HDL-C, decreased aortic integrin αVβ3 and YAP, and increased p-YAP (statistical significance reported). - Combined therapy advantages: The combined group showed larger weight reduction, greater decreases in TC, TG, proinflammatory cytokines, and aortic integrin αVβ3 and YAP expression, higher HDL-C, and increased p-YAP versus either monotherapy (P values reported in source). - Mechanistic implication: The authors conclude the combined intervention has synergistic effects on lipid profile, endothelial function, and inflammation in AS mice, potentially mediated by modulation of the **integrin/YAP/JNK** signaling axis in the aorta. - Safety/conflict: The source reports no conflicts of interest. Detailed adverse-event or safety data were not reported in the abstract.
## Clinical Analysis & Structured Key Points
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Affiliations Expand ### Affiliations * 1 Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China. * 2 Acupuncture and Tuina College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075. * 3 The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550002. llewshaw1@163.com. * 4 Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China. cuijin@gzy.edu.cn. * PMID: **42621708** * DOI: [ 10.13702/j.1000-0607.20250861 ](https://doi.org/10.13702/j.1000-0607.20250861) Free article Item in Clipboard # [Mechanism of herbal cake-separated moxibustion combined with atorvastatin in treating ApoE-/- atherosclerotic mice based on the integrin/YAP/JNK signaling pathway] [Article in Chinese] Hong-Ying Li et al. Zhen Ci Yan Jiu. 2026. Free article Show details Display options Display options Format Abstract PubMed PMID Zhen Ci Yan Jiu Actions * [ Search in PubMed ](https://pubmed.ncbi.nlm.nih.gov/?term=%22Zhen+Ci+Yan+Jiu%22%5Bjour%5D&sort=date&sort_order=desc) * [ Search in NLM Catalog ](https://www.ncbi.nlm.nih.gov/nlmcatalog?term=%22Zhen+Ci+Yan+Jiu%22%5BTitle+Abbreviation%5D) * [ Add to Search ](https://pubmed.ncbi.nlm.nih.gov/42621708/) . 2026 Aug 25;51(8):978-990. doi: 10.13702/j.1000-0607.20250861. ### Authors [Hong-Ying Li](https://pubmed.ncbi.nlm.nih.gov/?term=Li+HY&cauthor_id=42621708)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42621708/#short-view-affiliation-1 "Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China."), [Hong-Yan Luo](https://pubmed.ncbi.nlm.nih.gov/?term=Luo+HY&cauthor_id=42621708)[ 2 ](https://pubmed.ncbi.nlm.nih.gov/42621708/#short-view-affiliation-2 "Acupuncture and Tuina College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075."), [Min Zhu](https://pubmed.ncbi.nlm.nih.gov/?term=Zhu+M&cauthor_id=42621708)[ 2 ](https://pubmed.ncbi.nlm.nih.gov/42621708/#short-view-affiliation-2 "Acupuncture and Tuina College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075."), [Kai-Yang Xue](https://pubmed.ncbi.nlm.nih.gov/?term=Xue+KY&cauthor_id=42621708)[ 1 ](https://pubmed.ncbi.nlm.nih.gov/42621708/#short-view-affiliation-1 "Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China."), [Xiao-Xiao Lu](https://pubmed.ncbi.nlm.nih.gov/?term=Lu+XX&cauthor_id=42621708)[ 3 ](https://pubmed.ncbi.nlm.nih.gov/42621708/#short-view-affiliation-3 "The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550002. llewshaw1@163.com."), [Jin Cui](https://pubmed.ncbi.nlm.nih.gov/?term=Cui+J&cauthor_id=42621708)[ 4 ](https://pubmed.ncbi.nlm.nih.gov/42621708/#short-view-affiliation-4 "Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China. cuijin@gzy.edu.cn.") ### Affiliations * 1 Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China. * 2 Acupuncture and Tuina College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075. * 3 The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550002. llewshaw1@163.com. * 4 Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China. cuijin@gzy.edu.cn. * PMID: **42621708** * DOI: [ 10.13702/j.1000-0607.20250861 ](https://doi.org/10.13702/j.1000-0607.20250861) Item in Clipboard Full text links Cite Display options Display options Format Abstract PubMed PMID ## Abstract in [ English, ](https://pubmed.ncbi.nlm.nih.gov/42621708/#eng-abstract) [ Chinese ](https://pubmed.ncbi.nlm.nih.gov/42621708/#zho-abstract) **Objectives:** To investigate the effects of herbal cake-separated moxibustion combined with atorvastatin on lipid metabolism, aortic pathological structure, plaque deposition, and integrin/ yes-associated protein (YAP)/ c-Jun N-terminal kinase (JNK) signaling pathway in ApoE-/- atherosclerosis (AS) mice, and to explore its preventive and therapeutic mechanisms for AS. **Methods:** Male C57BL/6J mice were used as the blank group, and male ApoE-/- mice were randomly divided into the model group, medicine group, herbal cake-separated moxibustion group, and combined treatment group, with 9 mice per group. High-fat diet was used to establish the AS model. The herbal cake-separated moxibustion group received moxibustion at "Danzhong" (CV17) and "Shenque" (CV8) acupoints, once every other day, 3 times a week. The medicine group received atorvastatin calcium tablet via gavage, 3 mg·kg-1·d-1, once daily. The combined treatment group received an integrated approach that combined the interventions of the medicine group and herbal cake-separated moxibustion group. All groups were treated for 8 weeks. Body weight were observed before and after treatment. HE staining was used to observe aortic pathological morphology, and Oil Red O staining to observe aortic lipid plaque area. Biochemical analysis was used to detect serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) levels. Serum vascular endothelial growth factor (VEGF), endothelin-1 (ET-1), interleukin (IL)-6, IL-8, and tumor necrosis factor-α (TNF-α) levels were detected by ELISA, and serum nitric oxide (NO) level was detected by colorimetric assay. Western blot was used to detect protein expression levels of integrin αVβ3, YAP, phosphorylated (p)-YAP, p-JNK1/2, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) in the aorta. **Results:** Compared to the blank group, the model group exhibited increased body weight (_P_ <0.01)；thickened aortic intima, foam cells and lipid deposition, hypertrophic edema of smooth muscle cells, thinning of elastic fibers, widened endothelial cell gaps, and rupture of elastic membranes；significantly increased arterial lipid plaque area (_P_ <0.01)；elevated serum TC, TG, LDL-C, IL-6, IL-8, TNF-α, ET-1, and VEGF levels (_P_ <0.01), while HDL-C and NO levels decreased (_P_ <0.01)；increased protein expression of integrin αVβ3, YAP, p-JNK1/2, ICAM-1, and VCAM-1 (_P_ <0.01), and reduced p-YAP protein expression (_P_ <0.01) in the aorta. Compared to the model group, the medicine group, herbal cake-separated moxibustion group, and combined treatment group showed reduced body weight (_P_ <0.01), with a significantly lower weight change difference than that of the model group (_P_ <0.01)；more regular aortic lumen structure, thinner intima, reduced foam cells and lipid deposition, alleviated smooth muscle cell edema, hypertrophy, and inflammatory infiltration；all other indicators also showed significant improvement compared to the model group (_P_ <0.01, _P_ <0.05). Compared to the herbal cake-separated moxibustion group, the medicine group and combined treatment group exhibited reduced plaque area (_P_ <0.05, _P_ <0.01), increased serum NO level (_P_ <0.01), decreased LDL-C, ET-1, and VEGF levels (_P_ <0.01), and reduced protein expressions of p-JNK1/2, ICAM-1, and VCAM-1 in the aorta (_P_ <0.05, _P_ <0.01)；the medicine group also showed decreased serum TC, TG, IL-6, IL-8, and TNF-α levels (_P_ <0.01), increased HDL-C level (_P_ <0.01), reduced integrin αVβ3 and YAP protein expressions in the aorta (_P_ <0.01, _P_ <0.05), and increased p-YAP protein expression (_P_ <0.05). Compared to the medicine group and the herbal cake-separated moxibustion group, the combined treatment group exhibited a greater reduction in body weight (_P_ <0.05, _P_ <0.01), decreased serum levels of TC, TG, IL-6, IL-8, and TNF-α, as well as reduced expressions of integrin αVβ3 and YAP proteins in the aorta (_P_ <0.01, _P_ <0.05), elevated serum HDL-C level (_P_ <0.01), and increased p-YAP protein expression (_P_ <0.01). **Conclusions:** Herbal cake-separated moxibustion combined with atorvastatin exerts synergistic effects, ameliorates serum lipid profiles, mitigates endothelial dysfunction of aorta, and attenuates inflammatory responses in AS mice, potentially via modulation of the integrin/YAP/JNK signaling pathway in the aorta. **目的** : 观察隔药饼灸联合阿托伐他汀对ApoE⁻/⁻动脉粥样硬化（AS）小鼠血脂代谢、主动脉病理结构、斑块沉积及整合素（integrin）/Yes相关蛋白（YAP）/c-Jun N末端激酶（JNK）信号通路的影响，探讨其防治AS的作用机制。**方法** : 雄性C57BL/6J小鼠作为空白组，雄性ApoE⁻/⁻小鼠随机分为模型组、西药组、隔药饼灸组和联合治疗组，每组9只。高脂饲料喂养复制AS模型。隔药饼灸组于“膻中”和“神阙”施以隔药饼灸，隔日1次，每周3次;西药组予阿托伐他汀钙片3 mg·kg-1·d-1，每日1次;联合治疗组联合运用西药组和隔药饼灸组的干预方法。各组均连续干预8周。治疗前后观察各组小鼠体质量，HE染色观察主动脉病理形态，油红O染色观察主动脉脂质斑块面积，生化分析仪检测血清甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）含量，ELISA法检测血清血管内皮生长因子（VEGF）、内皮素-1（ET-1）、白细胞介素（IL）-6、IL-8 和肿瘤坏死因子-α（TNF-α）含量，比色法检测血清一氧化氮（NO）含量，Western blot法检测主动脉integrin αVβ3、YAP、磷酸化（p）-YAP、p-JNK1/2、细胞间黏附分子-1（ICAM-1）、血管细胞间黏附分子-1（VCAM-1）的蛋白表达水平。**结果** : 与空白组相比，模型组体质量增加（ _P_ <0.01）;主动脉内膜增厚，泡沫细胞和脂质堆积，平滑肌细胞增生水肿，弹性纤维变薄，内皮细胞间隙增宽，弹性膜发生断裂;动脉脂质斑块面积显著增加（ _P_ <0.01）;血清TC、TG、LDL-C、IL-6、IL-8、TNF-α、ET-1、VEGF含量升高（ _P_ <0.01），HDL-C和NO水平降低（ _P_ <0.01）;主动脉integrin αVβ3、YAP、pJNK1/2、ICAM-1、VCAM-1蛋白表达增加（ _P_ <0.01），p-YAP蛋白表达减少（ _P_ <0.01）。与模型组相比，西药组、隔药饼灸组和联合治疗组小鼠体质量减轻（ _P_ <0.01），且体质量变化的差值明显低于模型组（ _P_ <0.01）;主动脉管腔结构较规则，内膜变薄，泡沫细胞和脂质沉积减少，平滑肌细胞水肿、肥大和炎性反应浸润减轻;其余各项指标也均较模型组有明显改善（ _P_ <0.01， _P_ <0.05）。与隔药饼灸组相比，西药组和联合治疗组斑块面积减小（ _P_ <0.05， _P_ <0.01），血清NO含量上升（ _P_ <0.01），LDL-C、ET-1和VEGF含量降低（ _P_ <0.01），主动脉p-JNK1/2、ICAM-1和VCAM-1蛋白表达减少（ _P_ <0.05， _P_ <0.01）;西药组血清TC、TG、IL-6、IL-8、TNF-α水平降低（ _P_ <0.01），HDL-C水平升高（ _P_ <0.01），主动脉integrin αVβ3、YAP蛋白表达减少（ _P_ <0.01， _P_ <0.05），p-YAP蛋白表达增加（ _P_ <0.05）。与西药组和隔药饼灸组相比，联合治疗组小鼠体质量减轻幅度更大（ _P_ <0.05， _P_ <0.01），血清TC、TG、IL-6、IL-8、TNF-α含量和主动脉integrin αVβ3、YAP蛋白表达降低（ _P_ <0.01， _P_ <0.05），血清HDL-C水平升高（ _P_ <0.01），p-YAP蛋白表达增加（ _P_ <0.01）。**结论** : 隔药饼灸联合阿托伐他汀具有协同增效作用，可调节AS小鼠血脂水平，减轻主动脉内皮损伤，降低炎性反应，其作用可能与调控主动脉integrin/YAP/JNK信号通路相关。. **Keywords:** ApoE-/- mice; Atherosclerosis; Herbal cake-separated moxibustion; Integrin αVβ3; Yes-associated protein; c-Jun N-terminal kinase. [PubMed Disclaimer](https://pubmed.ncbi.nlm.nih.gov/disclaimer/) ## Conflict of interest statement All authors declare that there is no conflict of interest. ## Similar articles * [ [Effect of "Wenyang Tongmai" moxibustion on endothelial function in ApoE-/- atherosclerotic mice via PI3K/Akt/mTOR signaling pathway]. ](https://pubmed.ncbi.nlm.nih.gov/42185058/) Zhu Z, Pan L, Yan ZB, Zhang N, Yang ZH, Xiong JJ, Yang XF.Zhu Z, et al.Zhen Ci Yan Jiu. 2026 May 25;51(5):583-592. doi: 10.13702/j.1000-0607.20250195.Zhen Ci Yan Jiu. 2026.PMID: 42185058Chinese. * [ [Effect of moxibustion on the SIRT1/FOXO3a signaling pathway in ApoE-/- mice with atherosclerosis]. ](https://pubmed.ncbi.nlm.nih.gov/38649205/) Yan ZB, Wu XM, Zhang N, Yang ZH, Zhu Z, Yu HM, Jia XQ, Yang XF.Yan ZB, et al.Zhen Ci Yan Jiu. 2024 Apr 25;49(4):376-383. doi: 10.13702/j.1000-0607.20230578.Zhen Ci Yan Jiu. 2024.PMID: 38649205Chinese. * [ [Mechanism of Tanyu Tongzhi Formula in treatment of atherosclerosis by maintaining vascular homeostasis based on TGF-β signaling pathway]. ](https://pubmed.ncbi.nlm.nih.gov/39805789/) Cui XS, Zhang HY, Chen YY, Li L, Gao JM, Hao W, Xie CZ, Liu JX, Fu JH, Guo H.Cui XS, et al.Zhongguo Zhong Yao Za Zhi. 2024 Dec;49(23):6429-6438. doi: 10.19540/j.cnki.cjcmm.20240802.709.Zhongguo Zhong Yao Za Zhi. 2024.PMID: 39805789Chinese. * [ [Effects of herbal-cake-separated moxibustion on the repair of vascular endothelial and SDF-1 in rabbits with atherosclerosis]. ](https://pubmed.ncbi.nlm.nih.gov/30942037/) Shen J, Liu T, Liu X, Ge JY, Wang HL, Guo AL, Liu ML, Chang XR.Shen J, et al.Zhongguo Zhen Jiu. 2019 Feb 12;39(2):173-8. doi: 10.13703/j.0255-2930.2019.02.017.Zhon
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