BackgroundThis study investigated whether peripheral blood expression of linear ubiquitin chain assembly complex (LUBAC) and OTU deubiquitinase with linear linkage specificity (OTULIN) is associated with stroke severity and functional outcome in acute ischemic stroke (AIS) patients.MethodsImmunofluorescence for LUBAC and OTULIN was performed in cortical autopsy specimens from two AIS cases. A total of 100 AIS patients and 100 age- and sex-matched healthy controls were enrolled.
Peripheral blood mRNA levels of OTULIN and LUBAC components, including HOIL-1 interacting protein (HOIP), heme-oxidized IRP2 ubiquitin ligase 1L (HOIL-1L), and SHANK-associated RH domain interactor (SHARPIN), were quantified using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Stroke severity at admission was assessed using the National Institutes of Health Stroke Scale (NIHSS).
Functional outcome was evaluated using the modified Rankin Scale (mRS) at 90 ± 7 days. Regression models were used to evaluate associations of HOIP and OTULIN with stroke severity and outcome after adjustment for confounders.
A HOIP × OTULIN interaction term was applied to assess effect modification.
Frontiers in Immunology published a clinical update in Infectious Disease on 20 May 2026.
The item focuses on Association of peripheral blood LUBAC and OTULIN expression with severity and outcome in acute ischemic stroke: a prospective cohort study.
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