by Ligabaw Worku, Amha Kebede, Ayalew Jejaw Zeleke, Saron Fekadu, Melat Abdo, Tigist Atele, Netsanet Worku, Mulugeta Aemero Background Anopheles mosquitoes are the main vectors of malaria. Effective vector control depends on understanding their species composition, behavior, distribution, and insecticide resistance.
This study investigated Anopheles species composition, susceptibility to deltamethrin, and the frequency of knockdown resistance ( kdr ) mutations in Maksegnit and Gendawuha, Northwest Ethiopia. Methods Anopheles larvae and pupae were collected from breeding sites during the rainy and post-rainy seasons and reared to adults under field insectary conditions following WHO guidelines.
In addition, adult mosquitoes were collected from houses near larval habitats. Only field-derived mosquito populations were used in this study.
Adult females (3–5 days old) reared from field-collected larvae were tested for susceptibility to 0.05% deltamethrin using WHO bioassays. Based on bioassay outcomes, mosquitoes were classified as phenotypically susceptible (died after exposure) or resistant (survived exposure), while field-collected adults represented an unexposed group.
A total of 480 mosquitoes (160 resistant, 160 susceptible, and 160 field-collected unexposed adults) were subjected to genomic DNA extraction.
PLOS ONE (Medicine) published a clinical update in Research Highlights on 05 Jun 2026.
The item focuses on Seasonal variation in species composition, deltamethrin susceptibility, and kdr mutations in anopheles mosquitoes in Northwest Ethiopia.
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