Whole Genome and Phylogenomic Insights into Vibrio Parahaemolyticus From Pacific White Shrimp

09 Apr 20264 min read0 viewsJournal Feed

GIST

Whole-genome sequencing of Vibrio parahaemolyticus isolates SU37A and SU91A from Pacific white shrimp was performed with Nanopore technology, followed by de novo assembly and annotation. The resulting genome profiles were high quality and revealed multiple antimicrobial resistance genes, including vanT, vanY, tet, adeF, and CARB-18, indicating resistance to diverse antibiotic classes.

Mobile genetic elements were found adjacent to key regulatory genes linked to antibiotic resistance, suggesting potential horizontal acquisition and dissemination of resistance traits among these strains. Virulence-associated genes identified predominantly relate to regulatory and stress-response functions such as rpoS, rpoE, crp, hfq, and arcA, which are implicated in adaptation to nutrient limitation, oxidative stress, and host immune pressures.

Functional subsystem analysis indicated substantial metabolic flexibility. Phylogenomic analyses positioned SU91A with a clinical strain (3148−98) and SU37A with a non-clinical strain (11−2), implying possible zoonotic potential for SU91A and environmental persistence for SU37A.

The study notes uncertainty where data are limited, but overall findings highlight resistance determinants and regulatory- and stress-related virulence factors that may underpin pathophysiology and environmental adaptation of V. parahaemolyticus in aquaculture contexts.

Clinical Editorial

Genomic Scope and Study Design

This work analyzes whole-genome sequences of Vibrio parahaemolyticus isolates SU37A and SU91A from Pacific white shrimp using Nanopore-based de novo assemblies to produce high-quality genome profiles.
Annotation focused on identifying antimicrobial resistance genes and virulence-associated features, with subsequent phylogenomic context to infer relatedness to known strains.

Antimicrobial Resistance Landscape

Multiple resistance determinants were identified, including vanT, vanY, tet, adeF, and CARB-18, suggesting resistance across several antibiotic classes.
Genomic context revealed mobile genetic elements positioned adjacent to key regulatory genes linked to antibiotic resistance, indicating possible routes for acquisition and dissemination of resistance traits among the strains.

Virulence and Adaptation Features

The majority of virulence-associated genes detected are tied to regulatory and stress-response pathways, notably rpoS, rpoE, crp, hfq, and arcA, which are implicated in adaptation to nutrient limitation, oxidative stress, and host immune challenges.
Functional subsystem analysis pointed to substantial metabolic versatility, supporting adaptability across varied environmental conditions.

Phylogenetic Context and Zoonotic Implications

Phylogenomics placed SU91A in proximity to the clinical strain 3148-98, whereas SU37A clustered with the non-clinical strain 11-2, a pattern that raises considerations about potential zoonotic characteristics for these aquaculture-associated isolates.

Context and Practical Relevance

parahaemolyticus can persist and adapt in aquaculture settings, with implications for pathophysiology during infection and the broader ecology of resistance and virulence factors.

The study contributes to understanding how V.
The findings underscore molecular factors that enable environmental persistence and potential cross-environment transmission, highlighting concerns for shrimp aquaculture and public health surveillance.

Limitations and Reporting Notes

The provided content does not specify experimental outcomes beyond genomic and phylogenomic findings, nor does it quantify resistance phenotypes or provide clinical or environmental prevalence data.