by Woong Sik Jang, Young Lan Choe, Soo Young Yoon, Chae Seung Lim, Min-Chul Cho Background Candida auris is an emerging multidrug-resistant yeast associated with invasive infections, healthcare-associated outbreaks, and high mortality, and is often misidentified by conventional diagnostic methods. Rapid, accurate, and scalable screening tools are essential for effective infection control, particularly in high-risk settings.
Materials and methods We developed a multiplex loop-mediated isothermal amplification (LAMP) assay that combines a broad-range Candida Pan target with a C. auris –specific target in a single isothermal reaction.
Assay conditions were optimized for primer ratio and temperature, and analytical sensitivity was evaluated using serial dilutions of culture-derived C. albicans and C.
auris DNA, as well as contrived specimens consisting of urine, swab, and whole-blood matrices. Clinical performance was assessed using 35 Candida -positive clinical specimens (blood, urine, ear swabs) and 94 non-infectious controls.
Results were compared with Candida Pan qPCR and C. auris qPCR.
Cross-reactivity was tested against common bacterial isolates. Results Under optimized conditions (1:1 primer ratio, 64 °C), the assay allowed species-level discrimination, with C.
PLOS ONE (Medicine) published a clinical update in Research Highlights on 24 Apr 2026.
The item focuses on Development of a Loop-Mediated Isothermal Amplification (LAMP) for the screening of Candida auris.
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