PNASPublic Health
Negative design enables cell-free expression and folding of designed transmembrane β-barrels
Summary
Proceedings of the National Academy of Sciences, Volume 123, Issue 15, April 2026. SignificanceMembrane proteins (MPs) are essential to biology and biotechnology yet designing them from scratch remains challenging. Using synthetic transmembrane β-barrels and a cell-free expression system with lipid vesicles, we show that conventional ...
Proceedings of the National Academy of Sciences, Volume 123, Issue 15, April 2026. SignificanceMembrane proteins (MPs) are essential to biology and biotechnology yet designing them from scratch remains challenging. Using synthetic transmembrane β-barrels and a cell-free expression system with lipid vesicles, we show that conventional ...