BackgroundThe regulatory mechanism of respiratory chain in Mycobacterium tuberculosis (M. tuberculosis) remains to be elucidated, and limited evidence about the effect of gene mutations in the respiratory chain on multidrug-resistant (MDR) isolates was reported.ObjectiveTo elucidated the effect of gene mutations in the respiratory chain on MDR isolates.MethodsWhole-genome sequencing was performed on analyzed strains of M.
tuberculosis. Random forest, gradient boosting decision tree and generalized linear mixed models were employed to identify mutations in respiratory chain genes that contribute to the phylogenetic clustering and development of MDR isolates.ResultsOverall, a total of 13402 isolates of M.
tuberculosis were included in the study. 4051 (30.09%) isolates showed MDR, and 1044 (7.76%) isolates were classified as single‐drug resistance (SDR).
The results showed that the single nucleotide polymorphisms (SNPs) of atpH A428G, cydA C942A, qcrA G181C, nuoF G66C, qcrB G1250T, nuoA G82C, and nuoG A1422G A1810G were significantly associated with phylogenetic clustering of MDR isolates.
Frontiers in Immunology published a clinical update in Infectious Disease on 20 May 2026.
The item focuses on Respiratory chain gene mutations associated with global phylogenetic clustering of drug-resistant Mycobacterium tuberculosis revealed by whole-genome sequencing.
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