Background and purposeInterferon-induced protein 27 (IFI27) is implicated in immune regulation, and regulatory T cells (Tregs) play a critical role in maintaining pulmonary immune homeostasis during sepsis. However, the relationship between IFI27 and Treg-mediated regulation in sepsis-associated lung injury remains unclear.
This study aimed to investigate the role of IFI27 in modulating Treg function during sepsis.MethodsPlasma IFI27 levels were measured in patients with sepsis and healthy controls. mRNA sequencing was performed to assess IFI27 expression profiles.
In vivo, IFI27 expression was examined in a cecal ligation and puncture (CLP) mouse model, and an IFI27 overexpression mouse model was used to evaluate its functional role in sepsis. Immunofluorescence staining and transmission electron microscopy were employed to assess ferroptosis in lung epithelial cells.
Flow cytometry was used to analyze Treg populations and their secretion of interleukin-10 (IL-10). In vitro, primary Tregs were co-cultured with mouse lung epithelial cells to determine the effects of IFI27 on IL-10 secretion in Tregs and epithelial ferroptosis.
Frontiers in Immunology published a clinical update in Infectious Disease on 22 Apr 2026.
The item focuses on IFI27-mediated regulation of regulatory T cells aggravates lung injury in sepsis via IL-10/STAT3 signaling.
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