Minimally invasive 1 mm skin biopsies capture site-specific transcriptomic heterogeneity in vitiligo

14 May 20264 min read0 viewsJournal Feed

GIST (Key Takeaways)

Background. Vitiligo is a chronic inflammatory skin disease characterized by clinical and molecular heterogeneity across lesional, perilesional and non-lesional skin within the same individual. Understanding these region-specific differences is essential for identifying early disease processes and developing targeted therapeutic strategies.
Skin biopsies represent a key approach to explore these differences, yet conventional 3–5 mm biopsies are invasive, requiring sutures, extended healing time and leaving visible scarring. Objectives. The objective of this study was to characterize region-specific transcriptomic alterations across lesional, perilesional, and non-lesional skin in vitiligo, using minimally invasive 1 mm skin punch biopsies.
Methods. In this study, bulk RNA sequencing was performed on 105 skin biopsies obtained from perilesional and (non-)lesional skin of non-segmental vitiligo patients, as well as healthy control skin. Differential gene expression was followed by pathway-level analyses, and Connectivity Map–based perturbational profiling was performed to predict candidate therapeutic compounds capable of reversing the lesional transcriptional signature.
Results. Transcriptomic profiling of 1 mm biopsies revealed disease-associated changes across lesional, perilesional, and non-lesional vitiligo skin, with distinct region-specific gene signatures reflecting immune activation and metabolic reprogramming.