Cell-dependent antithrombotic effect of tranexamic acid

BackgroundTranexamic acid (TXA) is a synthetic lysine analog that inhibits fibrinolysis by blocking lysine-binding sites on plasminogen and plasmin. Although early therapeutic TXA reduces bleeding mortality in major trials, prophylactic benefit appears context- and timing-dependent.

TXA is generally not associated with increased thromboembolism, yet its net effect on thrombus formation remains uncertain. Because plasmin(ogen) also acts on leukocytes, endothelium and platelets via cell-surface receptors, TXA may exert cell-dependent effects beyond antifibrinolysis.ObjectivesTo determine how cellular elements modulate TXA’s impact on thrombus formation.MethodsWe studied TXA in an in vivo murine venous thrombosis model without endothelial injury (IVC stenosis).

Plasma VWF: Ag and MCP-1 were measured by ELISA. Thrombin generation was assessed in whole blood and plasma-based systems to evaluate cell dependence.

Leukocyte-associated plasminogen activation was quantified using plasmin generation assays in fibrin. Primary hemostasis was assessed by tail bleeding.ResultsTXA reduced the odds of venous thrombus formation by 90% but did not alter thrombus mass once clots formed.

VWF: Ag remained unchanged, whereas the stenosis-induced rise in MCP-1 was largely suppressed by TXA.