Identification and clinical implications of immune-related hub genes in psoriasis

20 Apr 20264 min read0 viewsJournal Feed

GIST (Key Takeaways)

by Yuzhen Sun, Ziguang Zhou, Yu Mao, Niu Liu, Yanfeng Li, Weiyuan Fang Background Psoriasis, a chronic inflammatory skin disease affecting 2–3% of the global population, is driven by dysregulated immune responses. Despite advancements in biologic therapies, treatment challenges persist due to high recurrence rates. This study aimed to identify immune-related hub genes and elucidate their clinical implications in psoriasis pathogenesis and therapy. Methods Multiple microarray datasets from psoriasis patients (GSE30999, GSE106992, GSE14905, GSE78097, and GSE117468) were obtained to identify immune-key genes by differential gene analysis and Weighted Gene Co-expression Network Analysis (WGCNA).
Subsequently, immune-related hub genes were identified using the Least Absolute Shrinkage and Selection Operator (LASSO) algorithm and Protein-Protein Interaction (PPI) networks, with further validation through Gene Set Enrichment Analysis (GSEA) and Receiver Operating Characteristic (ROC) curves to assess exploratory within-sample discrimination. Pearson correlation analysis evaluated the relationship between hub genes, skin lesion severity, and treatment outcomes. The study also conducted immune infiltration by using the Cell-type Identification by Estimating Relative Subsets Of RNA Transcripts (CIBERSORT) algorithm and identified potential therapeutic targets by the Drug-Gene Interaction Database (DGIdb). Results Thirty-one immune-related key genes were identified, and six hub genes ( CLEC7A, CXCL1, IRF1, S100A12, S100A8, S100A9 ) were validated as central players in immune signaling pathways.
These genes exhibited within-sample discrimination (AUC > 0.9) and correlated with disease severity and biological therapy efficacy. Immune infiltration analysis revealed increased activated memory CD4 + T cells and M1 macrophages in lesional skin, which was strongly associated with hub gene expression. Additionally, drug-gene interaction analysis identified potential therapeutic agents targeting these genes. Conclusion This study identified six immune-related hub genes that were closely linked to the severity of psoriasis, the effectiveness of biological treatments, and infiltrated activated memory CD4 + T cells and M1 macrophages.
Our findings elucidate a novel immune-related hub gene network in psoriasis and provide potential targets for the development and application of biologics.

Clinical Editorial

PLOS ONE (Medicine) published a clinical update in Research Highlights on 20 Apr 2026. The item focuses on Identification and clinical implications of immune-related hub genes in psoriasis. Open the detail page to review the full original feed content.